Objective:To investigate the mechanism of the role of transcription factor C/EBP-β in the learning and memory impairment of Alzheimer's disease(AD)model mice induced by high-salt diet.Method:Experimental mice were divided into APP/PS1-ND group,APP/PS1-HSD group,APP/PS1/C/EBPβ+/--ND group,APP/PS1/C/EBPβ+/--HSD group,APP/PS1/C/EBPβ-HDO-ND group,APP/PS1/C/EBPβ-HDO-HSD group,15 in each group,were given normal diet or high-salt diet,and their learning and memory levels were detected by novel object recognition test,contextural fear conditioning test and cued fear conditioning test.The mRNA level of CEB-PB in hippocampal brain region was detected by fluorescence quantitative PCR,and the transcriptional activity of CEBPβ was detected by specific enzyme activity kit.Results:There was no significant difference between APP/PS1-HSD group and APP/PS1-ND group in NOR T2 test(P＞0.05).The differentiation index of APP/PS1-HSD group in NOR T3 test,percentage of freezing time of contextural fear conditioning test and percentage of freezing time of cued fear conditioning test were significantly lower than those of APP/PS1-ND group(P＜0.01).There were no significant differences between APP/PS1/C/EBPβ+/--ND group and APP/PS1/C/EBPβ+/--HSD group in NOR T2 and T3 tests,percentage of freezing time of contextural fear conditioning test and percentage of freezing time of cued fear conditioning test(P＞0.05).There were no significant differences between APP/PS1/C/EBPβ-HDO-ND group and APP/PS1/C/EBPβ-HDO-HSD group in NOR T2 and T3 tests,percentage of freezing time of contextural fear conditioning test and percentage of freezing time of cued fear conditioning test(P＞0.05).Com-pared with APP/PS1-ND group,C/EBPβ mRNA level and C/EBPβ activity in hippocampus of APP/PS1-HSD group were significantly increased(P＜0.01).There was a significant negative correlation between hippocampal C/EBPβ activity and cognitive function in APP/PS1 mice(r=-0.6215,P＜0.05).Conclusion:C/EBPβ is involved in cognitive impairment of Alzheimer's disease mediated by high-salt diet,which may be a new therapeutic target for Alzheimer's disease.
维普
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