Objective:To preliminary investigate the impacts of Crocin on the proliferation and apoptosis of fi-broblasts from pathological scars by regulating Hippo signaling pathway.Method:Human keloid fibroblasts(HKF)were isolated and cultured in vitro,and identified by immunofluorescence assay.The inhibition rate of cell prolifera-tion was calculated by MTT method,and the optimal intervention concentration of Crocin was screened.The ex-pression of YAP and TAZ mRNA was detected by qRT-PCR method,cell proliferation and apoptosis were detected by EdU staining,and flow cytometry,respectively.Western blot was applied to detect the expression of prolifera-ting cell nuclear antigen(PCNA),Bcl-2 associated X protein(Bax),cell anti apoptotic factor B cell lymphocyte tumor 2(Bcl-2),and Hippo pathway protein.Results:Crocin inhibited the proliferation of HKF cells in a concen-tration-dependent manner,IC50=111.56μmol/L.The positive rates of EdU in control group,Crocin low,medium and high dose groups were(39.56±2.45)％,(31.42±2.17)％,(23.81±1.79)％,(14.37±1.12)％,respec-tively.The apoptosis rates were(8.67±1.06)％,(14.85±1.12)％,(23.72±1.35)％,(31.47±2.64)％,re-spectively.Compared with the control group,the expression levels of YAP,TAZ mRNA,EdU positive cell rate,the expression levels of PCNA,Bcl-2,p-YAP/YAP,and TAZ proteins in HKF cells in Crocin low,medium,and high dose groups were obviously decreased,the apoptosis rate and the expression level of Bax protein were increased(P＜0.05).After overexpression of YAP/TAZ,the inhibitory effect of crocin on the proliferation of HKF cells and the promoting effect on the apoptosis of HKF cells were significantly weakened(P＜0.05).Conclusion:Crocin may inhibit the proliferation of HKF cells and promote their apoptosis by inhibiting the YAP/TAZ signaling pathway.
CrocinHippo signal pathwayPathological scarsFibroblastsProliferationApoptosis
维普
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