Objective:To measure the serum levels of monocyte chemotactic protein 1(MCP-1)and soluble fms-like tyrosine kinase receptor 1(sFlt-1)in patients with chronic glomerulonephritis,and to analyze their rela-tionship with renal function and prognosis.Method:From January 2018 to March 2020,122 patients with chronic glomerulonephritis were regarded as the research group,and 128 healthy individuals who underwent physical exami-nation in our hospital were regarded as the control group.Patients with chronic glomerulonephritis were divided into group A(normal renal function),group B(mild to moderate renal impairment),and group C(severe renal impair-ment)according to renal function.According to the results of follow-up,the patients were grouped into renal failure group(22 cases)and disease remission group(100 cases).The levels of MCP-1 and sFlt-1 were detected by enzyme-linked immunosorbent assay.The levels of blood urea nitrogen(BUN)and serum creatinine(Scr)of all subjects were detected by an automatic biochemical analyzer,glomerular filtration rate(eGFR)was estimated using the chronic kidney disease epidemiology collaborative research formula(CKD-EPI).The correlation of MCP-1,sFlt-1 with BUN,Scr,eGFR was analyzed by Pearson method.Receiver operating characteristic(ROC)curve was applied to evaluate the value of serum MCP-1 and sFlt-1 levels in predicting the prognosis of patients with chronic glomeru-lonephritis.Results:Compared with the control group,the levels of MCP-1,sFlt-1,BUN and Scr in the study group were higher(P＜0.05),and the eGFR was lower(P＜0.05).The levels of BUN,Scr,MCP-1 and sFlt-1 in group C were significantly higher than those in groups A and B(P＜0.05),and the levels of BUN,Scr,MCP-1 and sFlt-1 in group B were significantly higher than those in group A(P＜0.05).Pearson correlation analysis showed that MCP-1 was positively correlated with BUN,Scr and sFlt-1(P＜0.05),and negatively correlated with eGFR(P＜0.05),sFlt-1 was positively correlated with BUN and Scr(P＜0.05),and negatively correlated with eGFR(P＜0.05).Compared with the disease remission group,the levels of serum MCP-1 and sFlt-1 of the pa-tients in the renal failure group were higher(P＜0.05).ROC analysis showed that the AUC of serum MCP-1 and sFlt-1 levels in predicting the prognosis of patients with chronic glomerulonephritis was 0.967 and 0.965,respec-tively,the AUC of MCP-1 combined with sFlt-1 in predicting the prognosis of patients with chronic glomerulone-phritis was 0.984,with a sensitivity of 100.00％and a specificity of 94.00％.Conclusion:The levels of serum MCP-1 and sFlt-1 in patients with chronic glomerulonephritis are obviously increased.MCP-1 and sFlt-1 can be used as potential biological indicators for prognosis evaluation.
维普
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