Objective:To investigate the potential relationship between polymorphisms of VDR gene(rs2239185,rs7975232,rs2525046,rs2228570),GSDMB gene(rs2305480)and bronchial asthma of children in hubei province.Method:158 asthmatic children(asthma group)and 156 healthy children(control group)were recruited in this study.The genotypes of multiple single nucleotide polymorphisms(SNPs)loci in VDR and GSDMB genes in the two groups were analyzed by Sequenom MassArray spectrometry chip microarray technology.The differences in the distribution of these SNPs between the two groups were analyzed,so as to screen for the susceptibility to asth-matic children in hubei province.Results:The frequencies of three genotypes(GG,AG and A A)in rs2305480 were 64.56％,4.43％and 31.01％in the asthma group,and 51.28％,8.97％and 39.74％in the control group,and the difference of GG genotypes between the two groups was statistically significant(P＜0.05).In asthma group,the frequencies of the alleles G and A in rs2305480 were 80.06％,19.94％,and 71.15％,28.85％in the control group.The difference in gene frequencies of allele A between the two groups was statistically significant(P＜0.05).Among asthmatic children with different genotype of rs2305480 in gene GSDMB,the WBC,neutrophils(NEU％),lymphocytecounts(LYM％)and ensinophils(EOS％)were not significantly different(P＞0.05).There was no significant difference in the distribution of rs2239185,rs7975232,rs2525046,rs2228570 in geng VDR between the asthma grounp and the healthy control grounp.Conclusion:The polymorphism of rs2305480 in GSDMB gene was associated with the susceptibility to childhood asthma in hubei province,while the SNPs of rs2239185,rs7975232,rs2525046,rs2228570 in geng VDR have no signficant correlation with childhood asthma in hubei prov-ince.
维普
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