Objective:To explore whether the flavonoid quercetin effectively regulates EMT-related molecular signals in MCF-7 in triple negative breast cancer(TNBC)cells.Method:Breast cancer cell line MCF-7 was treated with 0,25,50,100μM quercetin and named control group,25μM group,50μM group,and 100μM group.The in-vasion and metastasis of cells were detected by Trans well,and the expression levels of EMT-related markers and protein phosphorylation levels of p38/GSK3β pathway molecules were detected by Western-blotting.Changes in cell invasion and metastasis were detected after small molecule inhibitors were applied to interfere with relevant path-ways.Results:Quercetin inhibited the migration and invasion of MCF-7 in a concentration dependent manner.The expression of E-cadherin was increased,while the expression of N-cadherin and Vimentin was decreased.Quercetin reduced protein phosphorylation levels of p38 and GSK3β pathway molecules in a concentration-dependent manner.The use of p38 and GSK3β inhibitors increased the expression of E-cadherin,decreased the expression of N-cadherin and Vimentin,and inhibited the migration and invasion of MCF-7.Conclusion:Quercetin can inhibit TNBC invasion and metastasis,mainly through inhibiting p38/GSK3β pathway to achieve the transition from EMT to MET.
QuercetinEpithelial-mesenchymalp38/GSK3β pathwayBreast cancer
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