Isolation of Oligomeric Proanthocyanidins from Grape Seed by TLC
The separation of Oligomeric Proantho cyanidins (OPC's) by TLCwas studied. After the experiments of various ascending elusions, the chromatography was carried out using an ascending elution with toluene/acetone/acetic acid (3∶3∶1). HPLC was also employed to analyze the compose of each fraction obtained from TLC.