Antioxidant peptides of Pleurotus ferulae lenzi were separated using ultra-filtration,ion-exchange and gel filtration chromatography,and its antioxidant properties were also studied in this study.Two kinds of peptides (＞ 10 000 polypeptide components and ＜10 000 polypeptide components)were isolated from the hydrolyzed solution of Pleurotus ferulae lenzi protein.The polypeptide components under 10 000 have higher · OH radical scavenging and DPPH· radical scavenging capacity than those above 10 000.After detaching ofpolypeptide components under 10 000 by DEAE-32,three elution peaks were obtained and P3 component showed higher hydroxyl scavenging capacity,reaching 50.22％.Five elution peaks at 220 nm were further isolated from P3 component by Sephadex G-25,and P3-2 component showed best capacity of ·OH radical scavenging,reaching 77.23％.According to standard equation,the molecular weight of P3-2 was about 1 862.The results provide a theoretical basis for further studies of active ingredient from Pleurotus ferulae lenzi in Xinjiang province.
Pleurotus ferulae lenziantioxidant peptidesseparation and purificationanti-oxidation
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NSTL
维普
万方数据
