Screening of Lyoprotectants and Optimization of High-Density Freeze-Drying Process for Bifidobacterium adolescentis
To investigate the most suitable lyophilization protection formula for Bifidobacterium adolescentis and improve the efficiency of industrial production,the authors examined the lyophilization protection properties of various saccharide and protein protectants for B.adolescentis,the protection effect of compounding saccharide protectants with different structures and the protective effect of that with protein.Finally,the ratio between the dried mush and the lyophilizing agent was optimized to achieve high-density lyophilization.The results showed that the protective effect of saccharide(alcohol)protectants on B.adolescentis was generally higher than that of protein lyophilization protectants,and the lyophilization protection effect of small molecule saccharide below tetrasaccharides was better,among which sorbitol and raffinose had the best effect,and the lyophilization survival rate increased to about 56％after the combination at the mass ratio of 1∶1.The lyophilization protection effects of the bacteria were not improved after compounding with protein,ascorbic acid,glutathione,trace elements,and other small molecules.Considering the practical production applications,sorbitol and raffinose were compounded with whey proteins to improve the looseness of the bacterial powder at the mass ratio of 3∶3∶2.The lyophilized survival rate of B.adolescentis was(77.08±5.20)％when the wet bacterial mass mixed with the protective agents at the ratio of 1∶1.2,and the total dry matter mass fraction of the sample was 25％.
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