基于16S rRNA基因测序分析烟叶发酵过程中表面微生物的多样性
Analyzing Microbial Diversity on Tobacco Leaves Surface During Fermentation Process Based on 16S rRNA Gene Sequencing
阴耕云 1肖冬 1王凯 1师建全 1朱玲超 1王明锋1
作者信息
- 1. 云南中烟工业有限责任公司,云南昆明 650231
- 折叠
摘要
雪茄烟叶经过发酵处理才能制成风味独特的雪茄产品,研究烟叶发酵过程中微生物的多样性对提高国产雪茄烟叶品质有重要意义.作者采集了来自云南省8个地区初步晾晒的雪茄烟叶和7种来自多米尼加共和国各地的优质雪茄烟叶,基于16S rRNA基因测序技术鉴定各个样品表面微生物种类,通过OTU聚类分析、Alpha多样性分析、物种组成分析等方法进行微生物多样性分析,获得晾晒烟叶表面的优势菌.结果显示,云南省雪茄烟叶的优势菌属为葡萄球菌属,其他常见菌属有假单胞菌属、无色杆菌属、棒状杆菌属、泛菌属等;多米尼加共和国雪茄样品中微生物物种分布较中国雪茄样品更均匀,微生物的多样性整体高于中国雪茄样品,其优势菌属多为葡萄球菌属、棒状杆菌属、四联球菌属;云南省临沧市雪茄烟叶优势菌属与多米尼加雪茄相似,而且它的物种多样性和均匀度高于中国其他地区,具有较大的发展潜力.
Abstract
Cigar tobacco leaves can be made into cigar products with unique flavors only after fermentation treatment.It is of great significance to investigate the diversity of microorganisms in tobacco fermentation process to improve the quality of domestic cigar tobacco.In this study,preliminarily air-cured cigar tobacco leaves from eight regions of Yunnan Province and seven high-quality cigar tobacco leaves from Dominican Republic were collected.The microbial species on the surface of each sample were identified based on 16S rRNA gene sequencing technology.Microbial diversity was analyzed by OTU clustering analysis,Alpha diversity analysis,species composition analysis,and other methods to obtain the dominant bacteria on the surface of preliminarily air-cured tobacco leaves.The results showed that the dominant bacterial genus in the cigar tobacco leaves of Yunnan Province was prevalently identified as Staphylococcus,while other common genera included Pseudomonas,Achromobacter,Corynebacterium,Pantoea,and so on.The distribution of microbial species in Dominican cigar samples was more uniform than that in domestic cigar samples,and the overall diversity of microbial species was higher than that of domestic cigar samples.The majority of their dominant bacteria genera were Staphylococcus,Corynebacterium,and Tetralogy.The dominant bacteria genera of cigar leaves in Lincang City of Yunnan Province were similar to those in Dominican cigar samples,and its species diversity and uniformity were higher than those in other domestic regions in China,indicating great development potential.
关键词
雪茄烟叶/16S/rRNA/多样性分析/优势菌群Key words
cigar tobacco leaves/16S rRNA sequencing/diversity analysis/dominant bacteria引用本文复制引用
基金项目
云南中烟科技项目(JB2022YL02)
云南中烟科技项目(2020CP01)
出版年
2024
NETL
NSTL
维普
万方数据