Transcriptomic Analysis of Breast Milk Extracellular Vesicles Promoting the Growth of Bifidobacterium lactis BB-12
Extracellular vesicles are one of the important functional components in breast milk,and previous studies in the author's lab have found that breast milk extracellular vesicles(BM-EVs)could significantly promote the growth of Bifdobacterium lactis BB-12(B.lactis BB-12).However,the specific mechanism is unclear.In this study,BM-EVs were extracted from human milk using the combination of ultracentrifugation and ultrafiltration methods,and were characterized by nanoparticle tracking analysis(NTA),transmission electron microscopy(TEM),and Western-blot analysis.Transcriptome data analysis was used to explore and identify the key genes of BM-EVs promoting the growth of B.lactis BB-12.The results showed that after BM-EVs intervention,the expression levels of ABC oligopeptide transport system-related genes pstC,pstA,metI in B.lactis BB-12 were significantly increased,as well as starch and sucrose metabolism-related genes BIF_02090,pentose phosphate pathway-related genes prsA and BIF_01321,D-amino acid metabolism-related genes murD and BIF_02122,and Glyoxylate and dicarboxylate metabolism-related gene pccA.In summary,BM-EVs may serve as a new type of prebiotic to promote the growth of Bifidobacterium in the intestine of infants,providing a theoretical basis for the development and application in next generation infant formula milk powder.
breast milkextracellular vesiclesBifidobacteriumgrowthtranscriptome
万方数据
维普
