基于多克隆抗体的糠氨酸ic-ELISA检测方法的建立
Development of ic-ELISA for Furosine Detection Based on Polyclonal Antibodies
杨敏仪 1罗雯倩 1贾荣业 1刘宇轩 1王荣智1
作者信息
- 1. 福建农林大学生命科学学院,福建福州 350002;福建农林大学生物农药与化学生物学教育部重点实验室,福建福州 350002;福建农林大学福建省病原真菌与真菌毒素重点实验室,福建福州 350002
- 折叠
摘要
采用甲醛法制备了糠氨酸完全抗原,通过交叉验证确保了多克隆抗体的特异性,从而建立了基于该抗体的糠氨酸间接竞争酶联免疫吸附测定(indirect competitive enzyme-linked immunosorbent assay,ic-ELISA)检测方法.结果显示,建立的ic-ELISA检测方法有效检测范围为12.20～2 143.42 ng/mL,最低检测限为2.69 ng/mL.该方法操作简便、迅速,成本低廉,同时具备高灵敏度和优异特异性,为高效检测食品中糠氨酸质量浓度提供了新的选择,具有一定实际应用价值.
Abstract
A complete furosine complete antigen was prepared using the formaldehyde method,and cross-verification was employed to ensure the specificity of the polyclonal antibodies.An indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)was successfully established based on the antibodies.The results demonstrated that the established ic-ELISA exhibited a detection range of 12.20 to 2 143.42 ng/mL,with a limit of detection(LOD)at 2.69 ng/mL.This method is simple,rapid,and cost-effective,offering high sensitivity and excellent specificity.It provides a novel option for the efficient detection of furosine in food,demonstrating its potential for practical application.
关键词
糠氨酸/动物免疫/多克隆抗体/ic-ELISAKey words
furosine/animal immunization/polyclonal antibodies/ic-ELISA引用本文复制引用
基金项目
福建省科技厅高校产学合作项目(2021N5009)
出版年
2024
NETL
NSTL
万方数据