Development of ic-ELISA for Furosine Detection Based on Polyclonal Antibodies
A complete furosine complete antigen was prepared using the formaldehyde method,and cross-verification was employed to ensure the specificity of the polyclonal antibodies.An indirect competitive enzyme-linked immunosorbent assay(ic-ELISA)was successfully established based on the antibodies.The results demonstrated that the established ic-ELISA exhibited a detection range of 12.20 to 2 143.42 ng/mL,with a limit of detection(LOD)at 2.69 ng/mL.This method is simple,rapid,and cost-effective,offering high sensitivity and excellent specificity.It provides a novel option for the efficient detection of furosine in food,demonstrating its potential for practical application.
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