Hypolipidemic Effect of Sargassum fusiforme Polysaccharide and Ultrasonic-Assisted Degradation on Hyperlipidemic Zebrafish
This study investigated the hypolipidemic effects of Sargassum fusiforme polysaccharide(SFPS)and ultrasonic-assisted degradation on both juvenile and adult zebrafish,serving as model organisms.Juvenile zebrafish were divided into control group,high-fat group,200 mg/L ultrasonic degradation of Sargassum fusiforme polysaccharide(SFPSUD)group(200-SFPSUD),600 mg/L SFPSUD group(600-SFPSUD),and 600 mg/L SFPS group(600-SFPS)with an experimental period of 24 h.Adult zebrafish were divided into control group,high-fat group,30 mg Sargassum fusiforme powder group(SFP),20 mg/L SFPSUD group(20-SFPSUD),50 mg/L SFPSUD group(50-SFPSUD),and 50 mg/L SFPS group(50-SFPS)with an experimental period of 28 d.In the experiment with juvenile zebrafish,the zebrafish in 600-SFPS group exhibited less accumulation of fat in the blood vessels and abdomen than those in the high-fat group,while the zebrafish in 600-SFPSUD group showed the least fat accumulation.In the experiment with adult zebrafish,compared to the high-fat group,the 50-SFPS group showed a decrease in the body fat change rate by 25.53％,while the activities of hepatic lipase(HL)and total lipase(TL)increased by 153.00％and 123.48％,respectively.The 50-SFPSUD group exhibited a decrease in the body fat change rate by 27.18％,with HL and TL activities increased by 168.20％and 137.73％,respectively.Hematoxylin-eosin staining(HE)demonstrated effective inhibition of fat accumulation in the liver of zebrafish in both the 50-SFPS and 50-SFPSUD groups,with the intervention of SFPSUD being more significant.The above results show that Sargassum fusiforme polysaccharide has the function of reducing blood lipid.Ultrasonic degradation can improve the hypolipidemic capability of Sargassum fusiforme polysaccharide.The mechanism may be to improve the activities of TL and HL of zebrafish,so as to accelerate the metabolic rate of lipid in vivo.This study can provide theoretical reference for the further development of SFPS and ultrasonic-degraded functional foods,and provide new solutions to issues such as human hyperlipidemia and obesity in cultured fish.
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