Hydrolyzed wheat protein was analyzed by basic physicochemical components,relative molecular mass distribution,and amino acid composition.The peptide composition and sequence were identified by LC-MS/MS.The results showed that the total protein mass fraction of hydrolyzed wheat protein was(80.53±8.06)％,with the relative molecular mass mainly below 2 000 and a mass fraction of essential amino acids approximately 52.4％.Eight peptides were identified by Q3 scanning,PIS scanning,and MRM quantification,including leucyl-glutamine(LQ),valanyl-glutamine(VQ),isoleucyl-isoleucine(Ⅱ),leucyl-leucine(LL),alanyl-isoleucine(AI),valanyl-leucine(VL),leucyl-methionine(LM)and isoleucyl-methionine(IM),with mass fractions of 385.0,32.5,30.0,122.8,8.9,5.9,134.9,147.0 μg/g,respectively.These peptides may exhibit functional activities in vivo and serve as the material foundation for the application of hydrolyzed wheat protein in nutritional health food.
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