食品与生物技术学报2024,Vol.43Issue(10) :12-20.DOI:10.12441/spyswjs.20230621004

牛乳清蛋白的合成生物学研究进展

Progress in Synthetic Biology of Bovine Whey Proteins

翟征远 侯凯旋 王怡
食品与生物技术学报2024,Vol.43Issue(10) :12-20.DOI:10.12441/spyswjs.20230621004

牛乳清蛋白的合成生物学研究进展

Progress in Synthetic Biology of Bovine Whey Proteins

翟征远 1侯凯旋 2王怡2
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作者信息

  • 1. 中国农业大学食品科学与营养工程学院,北京 100083;中原食品实验室,河南 漯河 462300
  • 2. 中国农业大学食品科学与营养工程学院,北京 100083
  • 折叠

摘要

牛乳清蛋白约占牛乳中总蛋白质质量的20%,因其较高的营养价值和多功能性,在食品工业中被广泛应用,市场需求巨大.但国内在牛乳清蛋白生产方面存在原料不足、缺乏高效分离技术等问题.传统生产方式不仅复杂,而且伴随严重的"三废"污染.利用合成生物学技术,通过建立微生物细胞工厂,能够实现食品蛋白质的大量合成.目前,这种异源蛋白质表达方法已逐渐用于牛乳清蛋白的生产.作者概述了牛源β-乳球蛋白和α-乳白蛋白的功能与应用,介绍了当前利用各种表达系统进行生物合成的现状,并对未来优化底盘细胞、开发更高效的表达元件,从而实现牛乳清蛋白的工业化生产进行了展望.

Abstract

Bovine whey protein accounts for about 20%of the total protein mass in cow's milk.With its rich nutritional value and multifunctionality,it is widely applied in the food industry and has substantial market demand.However,domestic production of bovine whey protein faces challenges such as insufficient raw materials and a lack of efficient separation technologies.Traditional production methods are complex and generate significant"three wastes"pollution.Recent advancements in synthetic biology have enabled the construction of microbial cell factories for the large-scale synthesis of food proteins.Currently,this heterologous protein expression technology has been gradually applied to bovine whey protein production.The functions and applications of bovine β-lactoglobulin and α-lactalbumin are outlined,the current status of biosynthesis using various expression systems is described,and the future optimization of chassis cells and development of more efficient expression components for the industrial production of bovine whey proteins are envisioned.

关键词

牛源β-乳球蛋白/牛源α-乳白蛋白/蛋白质异源表达/细胞工厂

Key words

bovine β-lactoglobulin/bovine α-lactalbumin/heterologous protein expression/microbial cell factories

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出版年

2024
食品与生物技术学报
江南大学

食品与生物技术学报

CSTPCD北大核心
影响因子:0.674
ISSN:1673-1689
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