To investigate the antioxidant activity of non-centrifugal sugar,tert-butyl hydroperoxide(t-BHP)-induced Caco-2 cells were used as the model of oxidative damage to assess the effects of non-centrifugal sugar crude extracts on cell viability,intracellular reactive oxygen species(ROS)levels,and cellular antioxidant activity(CAA).Additionally,high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)was employed to analyze the main active phenolic compounds in non-centrifugal sugar.The results demonstrated that the non-centrifugal sugar extract could alleviate apoptosis induced by t-BHP,significantly reduce intracellular ROS levels,and exhibit notable antioxidant activity.The CAA value of the non-centrifugal sugar extract was equivalent to 12.27 μmol quercetin per gram of extract.Moreover,the non-centrifugal sugar extract contained phenols substances such as protocatechuic acid,vanillic acid,chlorogenic acid,cyanidin chloride,coumarin,and apigenin.This study provides a theoretical foundation for exploring the bioactivity of non-centrifugal sugar and its extracts,as well as the development of related functional products.
关键词
细胞试验/氧化应激/酚类化合物/活性氧检测/细胞抗氧化活性
Key words
cellular assays/oxidative stress/phenolic compounds/reactive oxygen species detection/cellular antioxidant activity
维普
万方数据