摘要
通过水蒸汽蒸馏法,提取成熟杉木和间伐杉木的精油,并分别对其化学成分进行气相色谱-质谱(GC-MS)分析,采用GC峰面积归一化法进行定量,其中成熟杉木精油鉴定出65种化合物,占该精油总量的81.377％,其主要成分为柏木醇(37.791％).间伐杉木精油鉴定出59种化合物,占该精油总量的91.724％,其主要成分为柏木醇(27.196％).轴向薄壁组织是储存杉木精油的主要组织,借助生物数码显微镜测定并分析2种杉木轴向薄壁组织比量,研究发现,成熟杉木的薄壁组织比量为0.821％,间伐杉木的薄壁组织比量为0.406％.杉木组织结构的差异性,会影响杉木精油的含量及分布.揭示杉木精油成份的差异性,可为其高效开发与满足不同产品需求提供科学依据.
Abstract
Essential oils extracted from mature and thinned Cunninghamia lanceolata using steam distillation were subjected to gas chromatography(GC)-mass spectrometry analysis of the chemical composition.Quantification was performed using the GC peak area normalization method.Sixty-five compounds were identified in the mature timber of C.lanceolata,accounting for 81.377％of the total essential oils,and the major constituent was cedrol(37.791％).A total of 59 compounds were identified in the thinning timber of the C.lanceolata,accounting for 91.724％of the total essential oils.The major constituent was cedrol(27.196％).The axial parenchyma is the main tissue for storing the essential oils in C.lanceolata.Using a biological digital microscope,we determined and analyzed the tissue proportion of the axial parenchyma in two species of C.lanceolata.The present study revealed that the average proportion of parenchyma was 0.821％and 0.406％in mature and thinned C.lanceolata,respectively.The differences in the C.lanceolata tissue structure affect the content and proportion of essential oils in C.lanceolata.Understanding the differences in the essential oil components of C.lanceolata provides a scientific basis for the efficient development of essential oils and meet different product needs.
维普
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