Rapid Determination of Antioxidant Enzyme Activity in Drosophila Melanogaster Induced by High-Sugar or High-Fat Diets Based on NIRS
To develop a rapid NIRS methodology for the measurement of antioxidant enzyme activity in Drosophila melanogaster under different diet conditions,240 Drosophila samples were collected under high-sugar or high-fat diets and the activity of two antioxidant enzymes,catalase(CAT)and superoxide dismutase(SOD),was determined by the thiobarbituric acid colorimetric method and hydroxylamine method,respectively.The near-infrared spectrum(NIR)of Drosophila extract was collected.A partial least squares(PLS)quantitative correction model was constructed based on NIR and two antioxidant enzyme activity reference values,and the effects of different spectral pretreatment methods and bands on the model performance were compared.The overall results had satisfactory prediction performance.For Drosophila induced by high-sugar diets,the correlation coefficient(RP)and root mean square error of prediction(RMSEP)of the PLS quantitative correction model were 0.982 and 2.34 U/gprot for CAT and 0.983 and 0.86 U/gprot for SOD,respectively;for Drosophila induced by high-fat diets,the RP and RMSEP of the PLS quantitative correction model were 0.976 and 3.01 U/gprot for CAT and 0.969 and 2.16 U/gprot for SOD,respectively.It was proved that NIRS could be successfully applied to rapidly detect the activity of antioxidant enzymes in Drosophila melanogaster.
万方数据
维普
