Identification of Common Bamboo Species in Universities in Wenzhou Based on DNA Molecular Marker and Morphology
Due to the high similarity in morphology of bamboo species,it is very difficult to accurately identify bamboo species by traditional morphological methods.To explore the applicability and accuracy of DNA molecular marker in the taxonomic identification of Bambusoideae,two stable and polymorphic sequences of chloroplast DNA non-coding regions(trpl32-trn L and rpl16 intron)were selected for sequence identification and phylogenetic analysis of bamboo species in two universities,Wenzhou.Coupled with morphology,these samples could be identified as 11 species belonging to 3 genera of Bambusoideae.The phylogenetic analysis showed that bamboo species of different genera were very strongly separately clustered.In terms of genetic distance,the genetic distance among different genera was far greater than that among different species.Moreover,only based on molecular marker,the combination of DNA sequences achieved a 100%identification rate at the genus level but only 27.3%at the species level.Our study shows that DNA molecular marker can accurately identify bamboo species at the genus level,and combined with the illustrated encyclopedia of bamboos,it can effectively improve the accuracy and efficiency of bamboo species classification.
DNA BarcodingBambusoideaeGenetic VariationMolecular IdentificationPlant Diversity
万方数据
维普
