代谢矿井CH4和CO气体的微生物提取及生长代谢特性
Microbial extraction and growth metabolic characteristics of microorganisms metabolizing CH4 and CO gases in coal mine
邓寅 1王彩萍 1邓军 1王宁1
作者信息
- 1. 西安科技大学 安全科学与工程学院,陕西 西安 710054;西安科技大学 陕西省煤火灾害防治重点实验室,陕西 西安 710054
- 折叠
摘要
为了降低采空区CH4和CO等可燃性气体的产生和蓄积,抑制可燃性气体爆炸,保障煤矿安全开采,采用生物治理方法,对矿井不同环境样品中的微生物进行了定向富集和驯化,利用高通量测序方法,分析了富集后的菌液生长特性以及菌群结构关系,通过分离和提纯,确定了代谢CH4和CO气体微生物的形态特征及其生长代谢特性.结果表明:唯一碳源方法可以显著提高目标微生物的富集程度,且不同样品中的优势菌群表现不一,富集后菌群的CH4 代谢率达到80%,CO代谢率超过95%;纯化后提取到2 株甲烷氧化菌(S2、T7)和3 株CO氧化菌(M1、M7、M13),并确定了微生物的生长周期,其中CO氧化菌在对数期内完成了CO气体代谢,CO代谢率超过95%,而CH4气体代谢伴随甲烷氧化菌的生长全过程,代谢相对缓慢,CH4 代谢率分别为76.1%、74.5%;此外,甲烷氧化菌利用了更多的CH4气体进行生长繁殖,表现出比CO氧化菌更高的菌液浓度、更高的O2消耗量以及更低的CO2产生量,但CO氧化菌具有比甲烷氧化菌更强的气体代谢能力.研究结果可为采空区CH4 和CO等可燃性气体的综合防治提供基础,对防止采空区因自燃而引发热动力灾害事故,确保煤矿安全绿色开采具有重要意义.
Abstract
In order to reduce the production and accumulation of combustible gases in goaf such as CH4 and CO and so on,restrain the explosion of combustible gases and ensure the safety of coal mining,the bioremediation method was adopted to enrich and acclimate the microorganisms in different environ-mental samples of the mines,the growth characteristics and community structure of bacteria after en-richment were analyzed by high-throughput techniques,and the growth metabolic characteristics of CH4 oxidizing bacteria and CO oxidizing bacteria were mastered after isolation and purification.The results show that the sole carbon source method can significantly improve the enrichment degree of target mi-croorganisms,and the dominant bacteria in different samples are different,the CH4 metabolic rate rea-ches 80%,and the CO metabolic rate exceeds 95%after enrichment.After purification,2 strains of CH4 oxidizing bacteria(S2,T7)and 3 strains of CO oxidizing bacteria(M1,M7,M13)are extracted,and the growth cycle of the microorganisms is determined.Among them,CO gas is basically metabolized by CO oxidizing bacteria in the logarithmic period,with the CO metabolic rate exceeding 95%,CH4 gas metabolism accompanied the whole growth process of CH4 oxidizing bacteria,and its metabolic process lagged behind that of the CO oxidizing bacteria,the CH4 metabolic rates of S2 and T7 are 76.1%and 74.5%,respectively.In addition,CH4 oxidizing bacteria uses more CH4 gas for growth and reproduc-tion,showing higher growth concentration of bacterial fluid,higher O2 consumption and lower CO2 pro-duction than CO oxidizing bacteria,but CO oxidizing bacteria has stronger gas metabolic activity than CH4 oxidizing bacteria.The research results can provide a basis for the comprehensive prevention and control of the combustible gases in goaf such as CH4 and CO,and it is of great significance to prevent the thermal power disasters caused by spontaneous combustion in goaf and to ensure the safety and green mining of coal mines.
关键词
CH4和CO氧化菌/微生物提取/唯一碳源/菌群结构/生长代谢特性Key words
CH4 and CO oxidizing bacteria/microbial extraction/sole carbon source/microbial commu-nity structure/growth metabolic characteristics引用本文复制引用
基金项目
国家自然科学基金项目(5217-4201)
陕西省杰出青年科学基金项目(2022JC-30)
新疆维吾尔自治区重点研发计划项目(2022B03031-1)
出版年
2024
国家科技期刊平台
NSTL
万方数据