首页|Simultaneous determination of telmisartan and amlodipine in human plasma by LC-MS]MS and its application in a human pharmacokinetic study

Simultaneous determination of telmisartan and amlodipine in human plasma by LC-MS]MS and its application in a human pharmacokinetic study

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A rapid and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) assay method has been developed and fully validated for the simultaneous quantification of telmisartan and amlodipine in human plasma. Carbamazepine was used as an internal standard. Analytes and the internal standard were extracted from human plasma by solid-phase extraction technique using Waters Oasis HLB 1 cm3 (30 mg) extraction cartridge. The reconstituted samples were chromatographed on a Hypurity advance C18 column (50mm × 4.6mm, 5 gm) using a mixture of acetonitrile -5 mM ammonium acetate buffer (pH-4.0) (50:50, v/v) as the mobile phase at a flow rate of 0.8mL/min. The calibration curve obtained was linear (r_〉0.99) over the concentration range of 2.01-400.06 ng/mL for telmisartan and 0.05 -10.01 ng/mL for amlodipine. Method validation was performed as per FDA guidelines and the results met the acceptance criteria. A run time of 2.5 min for each sample made it possible to analyze more than 400 human plasma samples per day. The proposed method was found to be applicable to clinical studies.

TelmisartanAmlodipineHuman plasmaSolid-phase extractionLC-MS/MSPharmacokinetics

Vasu Babu Ravi、Jaswanth Kumar Inamadugu、Nageswara Rao Pilli、Vudagandla Sreenivasulu、Venkateswarlu Ponnerid

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Research Studies, Rayalaseema University, Kurnool 518002, India Wellquest Clinical Research Laboratories, Ramanthapur, Hyderabad 500013, India

Wellquest Clinical Research Laboratories, Ramanthapur, Hyderabad 500013, India

BioPolymer and Thermophysical Lab, Department of ChemisCry, Sri Venkateswara University, Tirupati 517502, India

Analytical and Environmental Chemistry Division, Department of Chemistry, Sri Venkateswara University, Tirupati 517502, India

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LC-MS/MS 高效液相色谱法 氨氯地平 替米沙坦 人血浆 药代动力学 同时测定 应用

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2012

西安医科大学学报(英文版)
西安交通大学

西安医科大学学报(英文版)

影响因子：0.07
ISSN：1671-8267
年,卷(期)：2012.2(5)
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