Relationship between leaf anatomical structures and drought resistance of newly introduced apple dwarf rootstocks
[Objective] The research was to study the antomical structures of 5 species apple drawf rootstocks to select the combination of strong drought-resistance of the stocks, which would provide a basis for cultivation and utilization of production and cross breeding of the selected parents. [Method] Experimental materials were 4 apple drawingstocks （F1 ,F2,F3 ,F4）which were introduced from France,and M26 （No. 5） which is one of the most widely used inter-stocks in agricultural production at present,and Malus prunifolia （Willd.） Borkh was grown two-year old as rootstock variety. Potted experiments were used to study dought index and anatomical structures of continuous soil drought stress and natural growth. The thickness of the upper cuticle, thickness of the low cuticle, leaf thickness, palisade tissue thickness, spongy tissue thickness, palisade tissue and spongy tissue ratio, tightness of leaf palisade tissue structure, looseness of leaf sponge tissue structure were measured by the way of permanent paraffin section and photics micro- scope photography,and drought resistance value of the 5 apple rootstocks combinations were calculated to compare by the method of membership functions. [Result] The combination 1 reached drought hazard of grade 5 after 40 d moderate moisture stress, combination 2 reached drought hazard of grade 5 after 24 d moderate moisture stress ; drought index of combination 1 was the lowest and combinations 2 was the highest after 40 d moderate moisture stress. Under water stress, combination 5 had the biggest thickness of upper cuticle,combination 3 had the smallest,the thickness of upper cuticle of combination 2 and 4 increased more,combination 3 dropped and combination 1 and 5 didn＇t change greatly compared with CK ; Combination 2 had the biggest thickness of low cuticle, combination 3 the smallest, the low cuticle combination 2 and 4 increased more,combination 3 and 5 dropped and combination 1 did not change largely compared with CK; combination 4 had the largest thickness of leaf, combination 3 was the smallest, the leaf thickness of conbination 1,3 and 5 dropped, combination 4 increased more and combination 2 did not change largely compared with CK; combination 4 had the biggest palisade tissue thickness, and combination 3 had the smallest, the palisade tissue thickness of combination increased more, combination 2 did not change largely, the rest of 3 all dropped compared with CK; the biggest spongy tissue thickness was combination 2, the smallest combination 1, and they increased slightly, the rest of 3 dropped compared with CK; palisade tissue and spongy tissue ratio of combination 1,4 and 5 increased more and the rest of 3 declined slighly compared with CK;both combination 1 and 4 had the large tightness of leaf palisade tissue structure and small looseness of leaf spongy tissue structure. [Conclusion] The order of drought resistance of the 5 combinations of apple rootstocks with membership functions is. 1〉4〉2〉5〉3.
apple dwarf rootstockanatomical structuresdrought resistancemembership functions
NETL
NSTL
万方数据
维普
