Identification of astaxanthin producer bacteria strain CHU-R and optimization of its fermentation conditions
【Objective】 The research identified the astaxanthin producer bacteria CHU-R,optimized the fermentation conditions,and laid the foundation for large-scale production of astaxanthin.【Method】 The strain CHU-R was identified by the physiology and 16S rDNA sequence.In terms of bacterial biomass of strain CHU-R and the astaxanthin production,CHU-R strains’nitrogen sources,carbon sources,microelement such as medium composition and temperature,initial pH,inoculation concentration,liquid volume,and sucrose content in culture conditions were optimized and optimal conditions for the orthogonal experiment were optimized to determine the best combination of medium.【Result】 CHU-R was Lactobacillus sp.nov strain.CHU-R strain in the medium suitable nitrogen source for ammonium and urea,carbon sources of sucrose and glucose,lack of Fe2＋ in the medium were not conducive to cell growth and astaxanthin accumulation.The optimal fermentation conditions of CHU-R for the inoculation concentration were as follows ≤50 mL/L,the liquid volume 50 mL/L,the initial pH 7.0-7.5,the fermentation temperature 26-30 ℃,fermentation time of 48-72 h.Under these conditions,strain CHU-R bacterial biomass and the astaxanthin production were higher.【Conclusion】 Lactobacillus sp.nov strain CHU-R in the optimization of fermentation conditions able to obtain higher yield of astaxanthin has a good application potential and economic value.
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NSTL
万方数据
维普
