首页|153种中草药对罗非鱼无乳链球菌和海豚链球菌的抑制活性研究

153种中草药对罗非鱼无乳链球菌和海豚链球菌的抑制活性研究

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[目的]研究中草药对罗非鱼无乳链球菌和海豚链球菌的抑菌活性,为生产中罗非鱼链球菌病害的防治提供参考.[方法]采用琼脂扩散法,测定153种中草药乙醇提取物对罗非鱼(Tilapia sp.)无乳链球菌(Streptococcus agalactiae)和海豚链球菌(S.iniae)的体外抑菌作用,并采用二倍稀释法测定中草药乙醇提取物的最小抑菌浓度(MIC)和最小杀菌浓度(MBC).[结果]153种中草药中,博落回(Macleaya cordata)、十大功劳(Mahonia fortunei)、三颗针(Berberis spp.)、紫草(Lithospermum erythrorhizon)、田七须(Panax notoginseng)、补骨脂(Psoralea coryliolia)、田基黄(Hypericum japonicum)和五倍子(Galla chinensis)提取物对无乳链球菌表现出了明显的抑制活性,其中博落回的抑菌效果最显著,抑菌圈直径为(25.1±0.6)mm;博落回、千斤拨(Flemingia philippinensis)、田七须、甘草(Bidens bi pinnata)、田基黄、败酱草(Patrinina villosa)、紫草、苦参(Sophora flavescens.)、补骨脂、北五味子(Schisandra chinensis)、五倍子、鬼针草(Bidens bi pinnata)、虎刺(Damnacanthus indicus)、三颗针、翠云草(B.bipin nata)、羌活(Notopterygium incisum Ting ex)、香薷(Elsholtzia patrini Garcke)对海豚链球菌具有明显的抑菌活性,其中博落回的抑菌作用最强,抑菌圈直径为(24.3±1.1) mm.对抑菌活性较强的中草药的MIC和MBC进行了测定,结果表明,三颗针和博落回提取物的抑菌和杀菌效果均较好.三颗针、博落回对无乳链球菌和海豚链球菌的MIC和MBC分别为0.31,0.63;0.63,1.25和1.25,2.50;0.94,1.88 mg/mL.[结论]博落回、紫草、田基黄、补骨脂、三颗针、田七须和五倍子对无乳链球菌和海豚链球菌均具有较好的抑菌效果.
The antimicrobial activities of 153 kinds of Chinese herbal medicines against the pathogen of Streptococcus from tilapia in vitro
[Objective] The antimicrobial effects of Chinese herbal medicines against the pathogen of Streptococcus from tilapia in vitro were studied. [Method] The antimicrobial effects of 153 Chinese herbal medicines were screened by disc agar diffusion. Double-dilution method was used to detect the minimal inhibitory concentration and the minimum bactericidal concentration of some herb medicines which had sig-nificant inhibitory effects to streptococcus,pathogens from tilapia streptococcusis,Streptococcus iniae and S. agalactiae. [Result] The results showed that 8 kinds of Chinese herbs including Macleaya cordata ,Ma-honia fortunei , Lithospermum erythrorhizon , Herberts spp. , Hypericum japonicum , Panax noto'ginseng , Psoralea corylifolia ,and Galla chinensie could effectively inhibit the growth of S. iniae,their minimal inhibitory concentrations and the minimal bactericidal concentrations were among 0. 31 - 20. 00 mg/mL and 0. 63 - 20. 00 mg/mL, respectively. Macleaya cordata had the best inhibitory effects with the diameter of inhibition zone(DIZ)of (24. 3 ± 1. 1) mm. Macleaya cordata ,Glycyrrhiza uralensi, Lithospermum erythrorhizon , Hypericum japonicum ,Panax notoginseng ,Flemingia philippinensis ,Patrinina villosa ,Sopho-ra flavescens , Psoralea coryli folia ,Schisandra chinensis ,Bidens bipinnata ,Selaginella uncinata ,Berberis spp,. and Galla chinensis inhibited the growth of S. agalactiae,their minimal inhibitory concentrations and the minimal bactericidal concentrations were 1. 25 - 20. 00 mg/mL and 2. 50 - 40. 00 mg/mL,respectively. Macleaya cordata had the strongest inhibitory effects with the DIZ of (24. 3 ± 1. 1) mm. Berberis spp. and Macleaya cordata were the two best medicines against Streptococcus iniae and S. agalactiae. The MIC and MBC of Berberis spp. were 0. 31,0. 63 mg/mL(Streptococcus iniae)and 1. 25,2. 50 mg/mL(S. agalactiae) while that of Macleaya cordata were 0. 63, 1. 25 mg/mL (Streptococcus iniae) and 0. 94, 1. 88 mg/mL (S. agalactiae). [Conclusion] The screening results confirmed the possible use of Chinese herbal medicines as a source of antimicrobial alternatives to control the streptococcus diseases in tilapia production.

Chinese herbal medicinesStreptococcus agalactiaeStreptococcus iniaeantimicrobiol activities

吴颖瑞、龚庆芳、方宏、梁万文、陈明、袁耀彬、何瑞杰

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中国科学院广西植物研究所广西植物功能物质研究与利用重点实验室,广西桂林541006

广西水产研究所,广西南宁530021

广西师范大学生命科学学院,广西桂林541000

中草药 无乳链球菌 海豚链球菌 抑菌活性

广西自然科学基金广西自然科学基金中国科学院广西植物研究所基本业务费项目中国科学院广西植物研究所基本业务费项目

桂科基0832021桂科攻1123006-4桂植业1000811009

2013

西北农林科技大学学报(自然科学版)
西北农林科技大学

西北农林科技大学学报(自然科学版)

CSTPCDCSCD北大核心
影响因子：0.893
ISSN：1671-9387
年,卷(期)：2013.41(1)
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