Effects of PEG-6000 stress on seed germination and seedling physiological characteristics of Gaillardia aristata Pursh
[Objective] The research was carried out to study the effects of polyethylene glycol (PEG-6000) stress on seed germination and seedling physiological characteristics of Gaillardia aristata Pursh.[Method] Using PEG-6000 to simulate drought stress,the change of seed germination rate,germination vigor and germination index of G.aristata Pursh together with the main root length,stem length,fresh weight,SOD activity and MDA content of the seedlings were studied with different weight concentrations of PEG-6000 (1％,2 ％,5 ％,10 ％,15 ％,20 ％ and 25 ％).[Result] With the increase of PEG-6000 concentrations,the germination vigor,germination rate and germination index of the G.aristata seeds decreased generally.When PEG-6000 concentration was ≥5 ％,the germination vigor significantly decreased.When it was ≥15％,the germination rate significantly decreased.When it was 25％,the seed could not germinate.The stem length and fresh weight of the seedlings decreased gradually,while the main root length increased initially (1％-10％) followed by decrease (10％-15％).SOD activity increased initially (1％-5％) and then decreased (5 ％-15 ％),while MDA content increased gradually throughout.[Conclusion] The larger the PEG-6000 concentration was,the more severely the seed germination and seedling growth of G.aristata were influenced,and the more deeply the cell membrane system of G.aristata was damaged.
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万方数据
维普
