Isolation and identification of wine related yeasts in Kunming
[Objective] This study focused on the isolation and identification of grape wine related yeasts in Kunming,Yunnan Province,to improve the screening of wine related yeasts and their subsequent use.[Method] Using grape fruits from Sanwa village,Guandu District,Kunming,sterile liquid SelMed enrichment medium was used to cultivate the yeasts from grape skin and monoclonal yeasts were obtained after dilution and culturing.ITS sequence analysis and RFLP analysis were used to identify related species,and microsatellites were used to access the different strains of Saccharomyces cerevisiae.[Result] 207 monoclonal isolates fingerprints were obtained from grape skin of Kunming,Yunnan.PCR and RFLP analysis revealed that these colonies belonged to 3 genera,Metschnikowia,Rhodotorula,and Saccharomyces.Metschnikowia was the richest genera,and 23 colonies belonged to S.cerevisiae.Microsatellite analysis showed that these S.cerevisiae colonies belonged to four strains,and one was commercial strain X5.[Conclusion] In vineyards of Kunming,there were at least five yeasts belonging to 3 genera,including S.cerevisiae,Rhodotorula mucilaginosa,Metschnikowia chrysoperlae,Metschnikowia pulcherrima,and Metschnikowia fructicola.Some of the isolated yeasts originated from Europe,which may be introduced to Kunming together with the grape plants.
NETL
NSTL
万方数据
维普
