Increasing immune efficacy of Newcastle disease vaccine in chicken by astragalus polysaccharide and oxymatrine
[Objective] This paper aimed to study the immune effect of astragalus polysaccharide (APS) and oxymatrine (OM) on Newcastle disease vaccine.[Method] Five hundred and fifty 14-day-old non-immunized Roman cocks were randomly divided into 11 groups.Groups 1 to 9 were OAP (OM and APS quality ratio of 6 ∶ 4),OM and APS at high (4 mg/mL),medium (2 mg/mL) and low (1 mg/mL) doses,respectively.Group 10 was vaccine control and group 11 was blank control.Groups 1 to l0 were vaccinated with NDV-Ⅳ at the ages of 14 and 28 days,respectively.At the same time of the first vaccination,the chickens in groups 1 to 9 were given compounds at high,medium and low doses and chickens in vaccination and blank control groups were given physiological saline by oral administration,once a day for three days.On days 14,21,28,35 and 42,peripheral T lymphocyte proliferations and serum antibody titers were determined with MTT method and β-microscopic method,respectively.On 14,21,28 and 35 days,the organ indexes of spleen and bursa were determined by killing 4 chickens in each group.[Result] T lymphocyte proliferations were promoted by OAP,OM and APS at high and medium doses and serum antibody titers of OAP at high and medium doses were significantly higher than those of vaccine control group.At any time,spleen and bursa indexes of OAP at all doses were significantly higher than those of vaccine control group (except the OAP low dose group for 35-day old chicken).[Conclusion] APS and OM could synergistically improve the immune efficacy of Newcastle disease vaccine in chicken.
Chinese herbal medicinal ingredients compoundsT lymphocyte proliferationantibody titerimmune organ index
NETL
NSTL
万方数据
维普
