Direct regenerated floral and vegetative buds from explants of Muscari armeniacum
[Objective] The study investigated the optimal tissue culture conditions for Muscari armeniacum to directly induce the regeneration of floral buds.[Method] Floral buds explants of M.armeniacum were used as materials.With 0.1 mg/L 2,4-D,the buds were treated with different concentrations of 6-BA (0,0.5,1,2,3,4 mg/L),GA3 (0,0.5,1,2,3,4 mg/L)and ZT (0,0.05,0.1,0.5,1,2 mg/L),respectively to select the optimal induction conditions for bud differentiation.Using MS + 2 mg/L 6-BA (or ZT) + 0.1 mg/L 2,4-D,effects of different explants including young floral bud,petal segment,pedicel,young leaf and callus on the optimal induction conditions of flower bud were also studied.[Result] The optimal induction conditions for bud differentiation were MS+2 mg/L 6-BA+0.1 mg/L 2,4-D.The optimal induction explant was floral bud with induction rate of 60.2 ％.With the same hormones conditions,pedicel,sliced pet als,leaf and callus explants can only be induced to form callus and vegetative buds.So the optimal induction explant is floral buds,optimal induction condition is MS+ 2 mg/L 6-BA+0.1 mg/L 2,4-D.[Conclusion]Optimal culture system for M.armeniacum to directly induce the bud differentiation was established.
Muscari armeniacumdirect regeneration of floral budsvegetative budstissue culture
NETL
NSTL
万方数据
维普
