Temporal and spatial expression characteristics of GPx5 in epididymis of sheep
[Objective] The study was conducted to explore the expression of glutathione peroxidase 5 (GPx5) in sheep epididymis at different developmental stages.[Method] The mRNA and protein expression levels of GPx5 in different parts of sheep epididymis at different developmental periods were determined using fluorescence quantitative PCR,immunoblotting and immunofluorescence methods.[Result] The quantitative real-time PCR results showed that the mRNA expression level of GPx5 at epididymis caput of young sheep was significantly higher than at other parts (P＜0.05).The mRNA expression level of GPx5 in adult sheep epididymis caput was significantly higher than that in lamb and elder sheep (P＜0.05).Western blot results showed that GPx5 protein was not expressed in epididymis of lamb.GPx5 was expressed in all parts of adult sheep epididymis and it was not expressed in epididymis cauda of elder sheep.The protein expression level of GPx5 was significantly higher at epididymis caput of adult sheep than at other parts (P＜0.05).The protein expression of GPx5 in epididymis caput of adult sheep was significantly higher than the expression in elder sheep (P＜0.05).Immunofluorescence results displayed that GPx5 protein positive signal was not detected in lamb epididymis.All parts of adult sheep epididymis had positive fluorescent signals,and the fluorescence intensities at epididymis caput and corpus were significantly higher than at epididymis cauda.Positive fluorescent signal was also found in liquid of epididymis tube.The epididymis caput and corpus in elder sheep had positive fluorescent signals and fluorescence was widely distributed in nucleus and cytoplasm of epithelium cell in epididymis duct.[Conclusion] The mRNA expression of GPx5 was detected in all developmental stages of sheep with significant difference in different parts.GPx5 protein distributed at the epithelium cell in epididymis duct but it was not expressed in epididymis of lamb and epididymis cauda of elder sheep.It was indicated that the protein expression of GPx5 was closely related with sexual development stages of sheep.
NETL
NSTL
万方数据
维普
