[目的]探究茯苓菊苣复合多糖(Poria cocos and chicory complex polysaccharides，PCCP)对二型糖尿病(T2DM)模型大鼠的降血糖及肝脏保护作用，为天然多糖产品的开发应用提供参考。[方法]制备茯苓菊苣复合多糖，测定其中的多糖含量。随机挑选10只SD大鼠作为正常对照组(CK1)，其余大鼠利用高脂饮食诱导联合腹腔注射低剂量链脲佐菌素(streptozotocin，STZ)建立二型糖尿病大鼠模型。将糖尿病模型大鼠随机分为模型对照组(CK2)和茯苓菊苣复合多糖低、中、高剂量组(LM、MM、HM)及阳性对照(二甲双胍)组(CK3)，每组10只，其中LM、MM、HM组分别每日灌胃茯苓菊苣复合多糖2。85，5。7，8。55 g/kg，CK3每日灌胃二甲双胍300 mg/kg，CK1和CK2每日灌胃等量生理盐水，连续灌胃4周。灌胃结束时检测大鼠体质量及餐后2 h血糖浓度、大鼠口服葡萄糖耐量(OGTT)和胰岛素耐量(ITT)，以及血清中胰岛素浓度、血脂指标(总胆固醇(T-CHO)、总甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)浓度)、肝损伤指标(肝脏系数和谷丙转氨酶(ALT)、谷草转氨酶(AST)活性)，并测定肝脏组织的氧化应激指标(超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化氢酶(CAT)活性)。显微镜下观察各组大鼠胰腺组织的病理变化。[结果]制备的茯苓菊苣复合多糖中多糖质量分数为(72。17±0。66)％。CK2大鼠组血糖与CK1组相比显著升高，MM组和HM组血糖与CK2组相比显著降低，体质量显著增加，肝脏系数显著降低。与CK2组相比，LM、MM、HM组大鼠胰岛素抵抗指数(HOMA-IR)显著降低，口服葡萄糖耐量和胰岛素耐量得到改善，AST和ALT活性显著降低;MM组T-CHO、TG、LDL-C浓度显著降低。显微观察结果表明，CK2组大鼠胰岛细胞数量明显减少，MM、HM组胰岛细胞数量有所恢复。肝组织氧化应激结果表明。CK2组大鼠SOD、CAT、GSH活性与CK1组相比显著降低，HM组SOD、CAT、GSH活性与CK2组相比显著升高。[结论]茯苓菊苣复合多糖具有降低二型糖尿病大鼠血糖的功效，对高血脂症有缓解作用，对肝脏和胰腺有保护作用。
Effects of poria cocos and chicory complex polysaccharides on blood glucose levels in type 2 diabetic rats
[Objective]In the present study,the hypoglycemic and liver protective effects of poria cocos and chicory complex polysaccharides(PCCP)were investigated in a type 2 diabetic rat model.This study provided a reference for the development and application of natural polysaccharide products.[Method]Preparation of PCCP,and determination of polysaccharide content in PCCP was conducted.Ten rats were randomly selected as the normal control group(CK1),and the rest of the rats were induced by with high-fat diet combined with intraperitoneal injection of low-dose streptozotocin(STZ)to establish a SD rat model of type 2 diabetes.The diabetes model rats were then randomly divided into the model control group(CK2),low,medium and high dose groups of PCCP(LM,MM,HM)and a positive control group(met-formin group,CK3)with 10 rats in each group.The LM,MM and HM groups were gavaged with 2.85,5.7 and 8.55 g/kg of poria and chicory complex polysaccharides,respectively,CK3 was gavaged with 300 mg/kg of metformin,and CK1 and CK2 were gavaged with the same amount of saline.The gavage process lasted for 4 weeks.The rats'body weight and 2 h postprandial blood glucose concentrations were at the end of the 4th week of gavage.Oral glucose tolerance test(OGTT)and insulin tolerance test(ITT)were performed at the end of the gavage.Subsequently,serum insulin concentrations,lipid indices:total choles-terol(T-CHO),total triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),and high-density li-poprotein cholesterol(HDL-C)concentrations,and liver injury indices:liver coefficient,alanine ami-notransferase(ALT),and glutamic oxalamine transaminase(AST)activities were measured in the rats.Indicators of oxidative stress:superoxide dismutase(SOD)activity,glutathione(GSH)activity,and cata-lase(CAT)activity)were detected in liver tissue.Pathological changes in the pancreatic tissues of rats in each group were observed under the microscope.[Result]The mass fraction of polysaccharide in PCCP(72.17±0.66)％.Blood glucose was significantly increased in CK2 group compared to CK1 group.Blood glucose concentrations and liver coefficient were significantly decreased in MM and HM groups compared to CK2 group.And body weight was significantly increased in MM and HM groups compared to the CK2 group.The insulin resistance index(HOMA-IR)was significantly reduced,oral glucose tolerance and insu-lin to-lerance were improved,while the activity of AST and ALT was significantly reduced in rats in LM,MM and HM groups compared to CK2 group.Additionally,T-CHO,TG,and LDL-C concentrations were significantly reduced in the MM group compared with the CK2 group.Morphological microscopic observa-tion of rat pancreatic tissue showed that the number of islet cells was significantly reduced in the CK2 group and recovered in the MM and HM groups.The results of oxidative stress in the liver tissue of rats showed that SOD,CAT and GSH activity were significantly reduced in CK2 group compared to CK1 group,and SOD,CAT and GSH activity were significantly increased in the HM group compared to CK2 group.[Conclusion]These results indicate that PCCP has a significant impact on reducing blood glucose concentrations in type 2 diabetic rats.Moreover,PCCP can effectively alleviate hyperlipidemia and also has a protective effect on the liver and pancreas in experimental animals.
万方数据
维普
