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庙台槭总DNA提取及鉴定

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木本植物体内酚类、多糖等次生物质含量较高,严重影响从中提取总DNA的产量和质量。针对这一问题探索出一种适合庙台槭总DNA的提取方法,并对提取的总DNA进行了纯度和浓度的鉴定,结果表明此方法可有效去除次生物质对DNA的干扰,样品DNA的质量和纯度较高,可用于随机引物RAPD扩增和随后的各种遗传学分析。
DNA extraction and characterization of Acer miaotaiense
he content of phenolic compound,polysaccharides and othersecondary metabolites is high in ligneous plant,which influences frequently the yield and quality of genome DNA when being extracted.In this study,a new method was introduced which do best for characterization the purity and concentration of genome DNA.The result showed that it could effectively eliminate the affections of the secondary materials to extracted genome DNA from Acer miaotaiense,and which be suitable for RAPD analysis and other genetic analysis

李珊、王玛丽、赵桂仿

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西北大学生命科学学院秦岭生物多样性研究中心,西安 710069

庙台槭 总DNA提取 RAPD

陕西省教育厅资助项目

00JK007

2001

西北植物学报
西北农林科技大学,陕西省植物学会

西北植物学报

CSCD
影响因子:1.031
ISSN:1000-4025
年,卷(期):2001.21(2)
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