Regulation of Salicylic Acid on Stomata Aperture of Epidermis in Cucumber Cotyledon
The influence of exogenous SA on stomata aperture of cotyledon epidermis was investigated by means of microscope, using cucumber seedling (Cucumis sativus L. cv. Zhongnong 203), the relationship between salicylic acid (SA) and stomata movement was analysed. The results revealed that stomata aperture gradually diminished accompany with concentration of SA increasing,and the effect of SA dissolved in phosphate buffer was similar to SA dissolved in distilled water. Meanwhile, stomata aperture became smaller along with the time of SA application prolonging,and there existed extremely significant (r=-0. 962* * ) or significant (r=-0. 914* ) negative correlation between stomata aperture and the time of application. Lower pH of solution enhanced the limited function of exogenous SA to stomata aperture,and higher concentration of SA was appeared more obvious. SA was the most effective at pH 6,at concentrations of 0.1 mmol/L (pH 8:90.2％ ;pH 7:93.8％ ;pH 6:96.3％) compared to the control at corresponding pH. The results suggested that exogenous application of SA to incubate epidermis of cotyledon or to roots in cucumber seedling could induced stomata closure,which was intensified by high concentration,long time,low pH of SA solution,and SA dissolved in distilled water appeared more effective compared to in phosphate buffer.
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万方数据
维普
