Spatiotemporal Expression of Five microRNAs and Their Target Genes during Flower Development of Treated Winter Buds of Grapevine in Growing Season
Quantitative RT-PCR was employed to analyze the expression of five important microRNAs (miRNAs), namely vv-miR160a, vv-miR171a, vv-miR159, vv-miR164c and vv-miR167c, and their target genes in winter buds growing on grapevine cv. Fujiminori's shoots with treatments of cut-back at various times. Results showed that the various treatments have different effects on the expression of miRNAs and their target genes, of which vv-miR160a, vv-miR171a and vv-miR159 showed distinct increases in expression in the winter buds differentiating to flower buds,and they showed the highest express level in inflorescences, while vv-miR164c and vv-miR167c exhibited obviously decreased expression and were lowly expressed in inflorescences. All the five miRNAs showed no distinct variation in the winter buds of control plants,indicating these miRNAs are involved in the second flower development of winter buds. The inverse expression trends between miRNAs and their target genes suggest that these miRNAs negatively regulate their target gene expression.
grapevinemicroRNAstarget geneswinter budsflower development expression
NETL
NSTL
万方数据
维普
