Effect of Elicitors on the Activity of L-Phenylalanine Transaminase in Plantlets of Thymus
The plantlets of thyme were cultured on the 1/2 MS containing different sucrose, the NO3 / NH4+ ,phenylalanine,glutamic acid and tyrosine,and were tested for the PAL activity. The PAL activities were also tested after the plantlets were induced for 0 -120 h by MeJA,SA and chitosan at different culture times (20 d,30 d,40 d). The results showed that the highest PAL activity was obtained when the plantlets of thyme were cultured on the 1/2 MS medium with 2% sugar concentration and 2 ·1 NO3~/NH4 + ratio for 30 d. The PAL activities were increased to 126. 3%, 150. 6% and 153. 9% compared with CK by adding 0.6 mmol/L phenylalanine, 0. 8 mmol/L glutamic acid, and 0. 4 mmol/L of tyrosine as precursor regulation,respectively. The PAL activities of plantlets cultured 20 d were increased 610. 3%,788. 1% and 592. 5% than that of CK by adding 1. 0 mmol/L MeJA,100 mg/L SA,and 200 mg/L chitosan as elicitors. The PAL activities of plantlets cultured 30 d were increased to 402. 4%, 541. 2% and 400. 1% compared with CK. And the highest PAL activities of plantlets cultured 40d were increased to 390. 2%,377. 1% and413. 4% than that of CK. Therefore,the PAL activity was affected by adding different inducer at differentculture time.
NETL
NSTL
万方数据
维普
