Identification and Genetic Variation Analysis of Castor Bean Hybrid by SSR
In order to identify the hybrid purity of castor bean in early seedling stage or before sowing,castor bean hybrid verification and genetic diversity of Fi seeds derived from CSR24證SR181 hybridization were performed by SSR markers. It was showed that the results by different loci were basically consistent, and the hybrid rates estimated by most loci were from 13. 46％ to 17. 27％, only which evaluated by RCM207 and RC129 were less than 10%. The variation was occurred on some loci of several individuals,4 progenies had non-parent markers and generated two new bands in primer RC242; the paternal special bands were absent and a new band was appeared in some progenies in primer Rco23 ,Rco26 ,Rco29 ,RC129, RCM613 and RCM999. The genetic diversity and cluster analysis of SSR data indicated that the distinct differentiation existed in F1 progenies,the genetic variation was obvious,and genetic similarity coefficient between parents and progenies was 0.45~1.0. It was provided a method for early verification of true hybrids and offered the theoretical reference for genetic analysis of offsprings.
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