首页|鸭儿芹肉桂酸4-羟化酶基因的克隆与不同温度下的表达分析

鸭儿芹肉桂酸4-羟化酶基因的克隆与不同温度下的表达分析

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为研究鸭儿芹木质素合成相关基因,以贵州省野生鸭儿芹为研究对象,克隆到1条1 631 bp长的肉桂酸4羟化酶(C4H)基因(CjC4H),并以鸭儿芹生长过程中主要面临的4种不同温度(10℃、18℃、30℃和38℃)处理0、0.5、1、2、4、8、12和24 h,考察鸭儿芹木质素合成基因CjC4H的表达情况,探讨鸭儿芹木质素合成基因受温度的调控关系,为鸭儿芹适宜生长温度的选择提供参考.开放阅读框(ORF)分析显示,CjC4H基因的开放阅读框(ORF)长度为1 521 bp,可编码506个氨基酸,蛋白分子质量为58.13 kD,等电点为9.38.进化树分析表明,同科植物之间C4H进化上相对保守.实时定量PCR检测鸭儿芹中CjC4H基因与温度的响应表达,结果表明38℃高温处理4h时,CjC4H基因表达量远高于其他温度处理;而10℃和18℃相对低温处理,CjC4H基因表达在24 h时明显提高.
Isolation and Expression Analysis of Cinnamic Acid 4-hydroxylase Gene under Different Temperatures in Cryptotaenia japonica Hassk
In order to study the lignin synthesis related genes of Cryptotaenia japonica Hassk,the gene CjC4H with 1 631 bp,which encoding the cinnamate 4-hydroxylase(C4H) involved into lignin synthesis,was cloned from wild variety C.japonica Hassk of Guizhou Province.C.japonica Hassk was grown under 4 different temperatures(10 ℃,18 ℃,30 ℃ and 38 ℃).The CjC4H genes expression was detected at 0,0.5,1,2,4,8,12 and 24 h to provide reference for C.japonica Hassk suitable growth temperature selec tion.The ORF(Open Reading Frame) length was 1 521 bp,which encoding 506 amino acids.The molecular weight of the enzyme of CjC4H was 58.13 kD,and the pI was 9.38.Phylogenetic analysis demonstrated that the enzyme of C4H relative conservative in different plant species.The CjC4H genes expression under different temperature detected by real time quantitative PCR,the results showed that the CjC4H gene expression under high temperature of 38 ℃ 4 h was much higher than that of other temperature treatments,and the expression of CjC4H increased significantly at 24 h under 10 ℃ and 18 ℃ low temperature treatments.

Cryptotaenia japonica HasskCjC4HLigninquantitative real-time PCRtemperature stressgene expression

谭国飞、王枫、王广龙、徐志胜、熊爱生

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南京农业大学作物遗传与种质创新国家重点实验室,农业部华东地区园艺作物生物学与种质创制重点实验室,园艺学院,南京210095

鸭儿芹 CjC4H 木质素 荧光定量PCR 温度处理 基因表达量

教育部新世纪优秀人才支持计划国家自然科学基金江苏省杰出青年基金江苏高校优势学科建设项目江苏省双创计划

NCET-11-067031272175BK201300272011PAPD2011JSSC

2014

西北植物学报
西北农林科技大学,陕西省植物学会

西北植物学报

CSTPCDCSCD北大核心
影响因子:1.031
ISSN:1000-4025
年,卷(期):2014.34(7)
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