Cloning and Expression Analysis of Zinc Finger Protein Gene FtLSD1 in Fagopyrum tataricum under Abiotic Stress
According to transcriptome data of Fagopyrum tataricum at flowering,using PCR and RT-PCR techniques,the DNA and full-length cDNA sequences of FtLSD1 gene (GenBank accession number:KP252134) were amplified from F.tataricum.The obtained sequences were analyzed by bioinformatics software,and the expression FtLSD1 gene were analysed by qPCR under UV-B,SA and 4 ℃ cold stress.The results showed that the DNA sequence of FtLSD1 gene was 2 427 bp,of which consisted 6 exons and 5 introns,in line with the principle of GU-AG splicing,and the cDNA of FtLSD1 contained a 528 bp ORF.The UV-B radiation and 2 mmol/L salicylic acid could lead to a significant increase in the expression of the FtLSD1 gene,while 4 ℃ cold stress remained stable expression levels of the gene.The results expected to lay a foundation for study the stress resistance in F.tataricum.
Fagopyrum tataricumzinc finger protein LSD1gene cloningabiotic stress
NETL
NSTL
万方数据
维普
