Genetic Diversity of Plant Allium tubiflorum and Allium neriniflorum Endemic to East Asia by ISSR Markers
Allium tubiflorum and A.neriniflorum (Allium L.) are two species whose perianth segments were connated at base into a tube.As typical representatives of the East Asian endemic plants,they are the ideal models to study the changes of temperate deciduous forest.Genetic diversity across 14 and 16 populations of A.tubiflorum and A.neriniflorum was analyzed using ISSR molecular markers.(1)At the species level,total percentage of polymorphic loci (PPB) of A.tubiflorum was 98.00％,Nei's gene diversity (H) was 0.264 8,and Shannon's information index (Ho) was 0.415 3.For A.neriniflorum.PPB,H and Ho were 95.56％,0.253 9 and 0.399 8,respectively,little lower than the values of A.tubiflorum.There were lower genetic variations at population level.The coefficient of genetic differentiation (Gst) of the two species were 0.421 8 and 0.430 1,and analysis of molecular variance (AMOVA) demonstrated that among populations component accounted for 38.95％ and 39.17％ of the total variation,and that most genetic diversity was present within population.(2)Combined the results of this study and previous cytology information,hybridization and polyploidization may be the evolutionay driving force of A.tubiflorum and A.neriniflorum.Meanwhile,the two species had asexual reproduction with bulb,to overcome fertility decline and lower number of individuals within population.Complex genetic background and diversity reproduction might be an important factor leading significant genetic differentiation within population.(3)The previous phylogenetic information and UPGMA analysis in this study showed A.tubiflorum and A.neriniflorum were sister groups and had close genetic relationship.Overlap regions of the distributions of the two species located between Taihang Mountains and Yan Mountains,and this regions may be their ancestor's distribution.There were abundant genetic variations among populations in the overlap regions.For this reason,the regions near Taihang Mountains and Yan Mountains might be the differentiated center and genetic diversity center of the two species.
Endemic to East AsiaA.tubiflorumA.neriniflorumISSRgenetic diversity
NETL
NSTL
万方数据
维普
