Cloning and Expression Analysis of Banana MaTLP1 Gene
Based on banana EST library, we cloned a cDNA of TLP protein from Musa acuminata subsp.malaccensis.This cDNA has an open reading frame of 942 nucleotides,which encodes a protein of 313 amino acids.Blast found that its sequence has a high identity with other thaumatin-like proteins(TLPs) and a specific conserved domain TLPs share was found by using Conserved Domain Search Service (CD Search) tool in NCBI and then we named it MaTLP1.Phylogenetic tree indicated that this protein has a close genetic relationship with Phoenix dactylifera.Moreover,it has similar evolutional pattern with M.acuminata subsp.malaccensis.Organ specific expression analysis showed that expression of MaTLP1 was high in roots, corms, cauloids and weak in leafs, flowers and fruits.Real-time PCR analysis showed that expression of MaTLP1 was highly increased in resistant banana cultivars against pathogen Fusarium oxysporum f.sp.cubense(Foc) and was fluctuated not obviously in susceptible banana cultivars related to resistant cultivars.These results revealed that MaTLP1 plays an important role in banana M.acuminate resistance against pathogen Fusarium oxysporum f.sp.cubense.
NETL
NSTL
万方数据
维普
