Clonging and Expression Analysis of γVPE Gene Related to Powdery Mildew Resistance in Grapevine
Vacuolar processing enzyme (γVPE) was cloned from Chinese wild Vitis pseudoreticulata accession.‘Baihe-35-1’ and Vitis vinifera cv.‘Carinena’ by RT-PCR.They were named VpγVPE and VvCγVPE.The full length of VpγVPE and VvCγVPE cDNA are 1 624 bp,and the open reading frame are 1 482 bp,encoding 493 amino acid.The alignment of the amino acid sequence revealed that Ser 395,one key amino acid in substrate pocket,was replaced by Ala in Vitis pseudoreticulata accession.‘Baihe-35-1’,Vitis vinifera cv.‘Carinena’,Vitis vinifera cv.‘Thompson seedless’,and Vitis vinifera cv.‘Pinot Noir’.The different expression profile of VpγVPE gene and VvCγVPE gene in different periods after powdery mildew induced were analyzed by Real-time fluorescent quantitative PCR.After induction the expression of VpγVPE gene increase slightly in 4 h,48 h,168 h and the VvCγVPE gene shows predominant expression in 4 h after that it decreases slowly.The study shows that γVPE is related to resistant to powdery mildew.The study provides reference value to the molecular mechanism of γVPE gene in disease resistance.
NETL
NSTL
万方数据
维普
