Effects of Physiological Characteristics of Cirsium japonicumLeaves on Simulated Drought Stress by PEG-6000
Take the seedlings of Cirsium japonicum as test materials, we studied the influence of relative water content (RWC), malondialdehyde (MDA) content, osmotic regulation substance contents and protective enzyme activities of leaves with PEG-6000 gradient concentrations(5%, 10%, 15%, 20%, 25%, 30%) under the stress time of 24 h, 48 h and 72 h to study the drought resistance and drought resistance mechanism preliminarily in the paper.The results showed that:(1)at stress of 24 h, 48h and 72 h,with the extension of stress time and the increase of treatment concentration, the relative water content showed a trend of decrease with the sharpest drop of 55.86%, while the MDA content significantly increased with the biggest increase of 186.21%.(2)With the extension of stress time, the soluble sugar and free proline contents of leaves increased under PEG-6000 ≤10%,while showed decrease trend after the first increase, and with the increase of treatment concentration the two indexes increased firstly and decreased afterward.The peak value of soluble sugar content appeared orderly at stress of 24 h, 48 h and 72 h on 20%, 20%,10% of PEG-6000 concentration, and the free proline appeared orderly on 20%, 15%, 15% of PEG-6000 concentration.At the stress 48 h, the above two indexes get the maximum increased nearly 4.7 times and 10.7 times on 20% and 15% of PEG-6000 concentration respectively compared with CK.(3)Expect SOD activity increased at stress 24 h, the protective enzyme activities all showed a trend of increase firstly and then decreased.The maximum increase was 370.14%, 248.91%and 118.78% respectively for SOD, POD and CAT activities.SOD and POD both appeared on stress 72h, 15% of PEG-6000 concentration, while CAT appeared on 48 h, 10% of PEG-6000 concentration.Above all, the C.japonicum has a stronger osmotic adjustment ability and higher enzyme activity with 15% of PEG-6000 concentration on a long time (72 h) stress, which shows a strong ability to drought tolerance.If over the concentration, the osmotic adjustment ability would be down, the enzyme activity weaken, the relative water content continuously reduced and the MDA content continuously increased.The physiological metabolism of C.japonicum was restrained.
Cirsium japonicumPEG-6000 stressthe relative water content of leafMDAosmotic adjustmentprotective enzyme
NETL
NSTL
万方数据
维普
