In this study,salicylic acid (SA),methyl jasmonate (MeJA),Ca2+,and sterile water as a control were used as exogenous inducers to pretreat the melons for resistance and susceptibility to fusarium wilt.Two days after the induction of pretreatment,they were inoculated with F.melilocarpa,and the incidence was observed on the 5,7 and 9 d after inoculation.The disease status was investigated,and melon leaves were taken at 1,3,5,7 and 9 d after inoculation.Expression changes of Fom-2 and CHT in resistant melon (MR-1) and susceptible melon (M1-15) leaves were evaluated in order to check the wilt in control wilt with the technical way of dyeing.The results showed that:(1) the disease indexes of the two varieties after inoculation with exogenous MeJA and SA were significantly lower than that of the control and there was no significant difference between the condition index after Ca2+ treatment and the control.(2) After inoculated with exogenously induced pretreatment,the expression of Fom-2 and CHT genes in leaves of MR-1 and M1-15 cultivars was different but the effect of Ca2+ on upregulation was not strong.(3) After inoculated with SA and MeJA,the expression of Fom-2 and CHT genes in the leaves of the two cultivars were significantly higher than those of the control,but the expression level of Fom-2 for resistant melon MR-1,the peaks were reached at 5 d and 7 d and all susceptible melon M1-15 were peaked at 9 d after inoculation.The expression of CHT gene for resistant melon MR-1 peaked at 7 d after inoculation.M1-15 showed peak at 7 and 9 days after inoculation,respectively.(4) There was no significant effect of Ca2+ treatment on the expression of Fom-2 and CHT genes in muskmelon leaves.(5) The correlation analysis showed that the disease index of Fusarium wilt was correlated with the expression levels of Fom-2 and CHT genes after SA and MeJA induced pretreatment inoculation.The effect of Ca2+ treatment is not significant.
NETL
NSTL
万方数据
维普
