Identification and Expression Characterization of HXK Gene Family in Potato(Solanum tuberosum)
In order to investigate the function of the potato HXK family,this study used bioinformatics to identify the members of the potato HXK gene family and real-time fluorescence quantitative PCR to ana-lyze the expression of these genes under different simulated stresses of adversity.This study identified six potato HXK genes,named StHXK1-StHXK6,from the potato genome using the homology matching method.The family genes were distributed on six chromosomes of potato,and their encoded proteins ranged from 496 aa to 533 aa in length,with an isoelectric point of 5.36-6.11 and molecular masses ran-ging from 53 736.38 D to 58 184.71 D,all of which were hydrophilic proteins.Subcellular localization pre-dictions indicated that members of the StHXK family were predominantly expressed in chloroplasts.The secondary structures of all members of this family were dominated by α-helices and irregular coiling,and the tertiary structures were highly similar.The promoter region of the StHXK gene contained light-re-sponsive elements,hormone-responsive elements,stress-responsive elements and growth and develop-ment-related elements.The qRT-PCR results showed that all StHXK genes were significantly up-regula-ted under ABA induction,and the relative expression of StHXK1 and StHXK2 was higher under 50μmol/L ABA stress at 24 h of treatment,286.4 times and 152.02 times higher than that of the control,respectively.Under 200 mmol/L NaCl treatment,the relative expression of all StHXK genes was down-regulated except for StHXK1 and StHXK4 genes which were up-regulated at 2 h of treatment time.The relative expression of StHXK genes was differentially up-regulated under 10％PEG stress treatment.In summary,the StHXK genes responded to ABA,NaCl and PEG stress treatments to varying degrees.
NETL
NSTL
万方数据
