Cloning and Function Analysis of MaMYB114 Gene in Grape Hyacinth
MYB transcription factors play a major regulatory role in plant anthocyanin biosynthesis.In or-der to explore the role of MYB transcription factor in the formation of the blue flower color of Muscari ar-meniacum,this study used M.armeniacum as the experimental material.Based on the local deep mining of the transcriptome database of the research group,authors cloned one R2R3-MYB gene named MaMYB1 14(GenBank accession number:OQ615377)for bioinformatics analysis and functional validation of heterologous transformed tobacco.The results showed that,(1)the open reading frame of MaMYB1 14 had a total length of 714 bp,encoding a protein of 237 amino acids.Sequence alignment showed MaMYB114 protein contains the R2R3 conserved domain,indicating that it belongs to the AN2 subfamily of the R2R3-MYB family.(2)The results of subcellular localization and transcriptional self-activation con-firmed that MaMYB1 14 was located in the nucleus and had transcriptional autoactivation activity,which was consistent with the general characteristics of transcription factors.(3)Real-time quantitative PCR analysis showed that MaMYB1 14 gene was highly expressed mainly in flowers,and the expression pattern was tissue-specific.Combined with the change patterns of anthocyanin content at different times,it was found that MaMYB1 14 was highly expressed in flower buds with high anthocyanin content during the colo-ring stage,indicating that MaMYB1 14 participated in the synthesis process of anthocyanins in grape hya-cinths.(4)Overexpression ofMaMYB114promotes anthocyanins accumulation in tobacco,indicating that MaMYB1 14 positively regulates anthocyanin synthesis.This study indicated that the MaMYB1 14 gene might be an important regulatory gene involved in the formation of the blue color of grape hyacinth.
Muscari armeniacumanthocyaninsMYB transcription factorheterologous overexpressiongene function
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