不同温度处理对珠芽魔芋球茎休眠调控的影响
Effects of Different Temperature Treatments on Dormancy Regulation of Konjac Bulbil
王蕊嘉 1李文涵 1赵青青 1谢雨琪 1李缘 1吴学尉1
作者信息
- 1. 云南大学资源植物研究院,昆明 650091
- 折叠
摘要
为探究不同温度处理对珠芽魔芋球茎休眠调控的影响,以珠芽魔芋叶面球茎为材料,在球茎休眠期设置昼/夜变温(24 ℃/9 ℃、24 ℃/13 ℃、24 ℃/17 ℃)、恒温(9 ℃、13 ℃、17 ℃、21 ℃)及室温处理,萌发期设置 26 ℃、33 ℃催芽处理,分析不同温度处理后珠芽魔芋球茎在休眠Ⅰ期、休眠Ⅱ期以及萌发前期、中期、后期、末期的生物表型变化、内源生理变化规律及其休眠调控相关基因的变化情况.结果表明,(1)休眠期恒温处理有利于提高珠芽魔芋球茎的出芽比,其中13 ℃恒温打破休眠和萌发期33 ℃催芽处理球茎的发芽率最先达到峰值,且其出芽比最高.(2)休眠Ⅱ期的球茎淀粉含量低于休眠Ⅰ期,而其可溶性糖含量高于休眠Ⅰ期,13 ℃恒温处理球茎淀粉含量下降最快,其可溶性糖含量最高;前期恒温处理球茎淀粉、可溶性糖含量在萌发前期和萌发后期之间均存在显著性差异,而前期昼夜变温处理球茎仅可溶性糖含量在萌发前期和萌发后期间差异显著.(3)珠芽魔芋球茎ABA含量在休眠Ⅰ期和休眠Ⅱ期逐渐增多,而其GA3含量逐渐减少,萌发期ABA含量均先升后降,同期GA3含量在26 ℃催芽处理下先升后降,在33 ℃催芽处理下整体上升.(4)珠芽魔芋NCED基因表达量随着休眠程度的加深逐渐增加,而在萌发期逐渐减少,CYP707A基因表达量随着休眠的加深逐渐减少,而在萌发期增加.研究发现,珠芽魔芋打破休眠的最佳温度为恒温13 ℃,促进萌发的最佳温度为33 ℃;随着珠芽魔芋打破休眠,球茎中的淀粉含量降低,可溶性糖含量升高,ABA含量先升后降,GA3含量先降后升;NCED和CYP707A基因可能是珠芽魔芋休眠调控中的关键基因.
Abstract
In order to explore the effects with different temperatures on the dormancy regulation of konjac bulbil,we used the foliar bulbils as the material.The day/night temperature(24 ℃/9 ℃,24 ℃/13 ℃,24 ℃/17 ℃)and constant temperature(9 ℃,13 ℃,17 ℃,21 ℃)were set during the dormancy period,and the temperature was set at 26 ℃ and 33 ℃ during the germination period.We analyzed the biological phe-notypic changes,endogenous physiological changes and expression of genes related to dormancy regulation after different temperature treatments in dormancy Ⅰ,Ⅱ,early germination,germination of mid,germi-nation of late and terminal germination stage.The results showed that,(1)constant temperature treat-ment during the dormancy stage was beneficial to improve the budding ratio of konjac bulbil.Dormancy was broken by constant temperature treatment at 13 ℃ in the dormancy stage,and germination rate of konjac bulbil reached its peak at 33 ℃ in the germination stage.(2)The content of starch in bulbil at dor-mancy Ⅱ stage was lower than at dormancy Ⅰ stage,the content of soluble sugar in bulbils at dormancyⅡ stage was higher than at dormancy Ⅰ stage.The content of starch in bulbils at 13 ℃ decreased the fas-test,and the content of soluble sugar in bulbils at 13 ℃ was the highest.There were significant difference in starch content and the soluble sugar content of bulbils between early and late stage of germination(P＜0.05).(3)The content of ABA in bulbils increased gradually,and the content of GA3 decreased gradually at dormancy stage Ⅰ and dormancy stage Ⅱ.The ABA content of bulbils in germination period showed a trend of"first rising and then decreasing"and the content of GA3 in bulbils treated at 26 ℃ also showed the same trend in germination period.The GA3 content of bulbils treated with a germination temperature of 33 ℃ showed an overall upward trend.(4)The expression level of NCED gene increased gradually with the deepening of dormancy and decreased gradually during germination;The expression of CYP707A gene decreased gradually with the deepening of dormancy and increased significantly during germination.In con-clusion,we found that the optimal temperature for breaking dormancy was 13 ℃,and the optimal temper-ature for promoting germination was 33℃.As the konjac bulbil broken the dormancy,the starch content in the bulbils decreased and the soluble sugar content increased;ABA content showed a trend of"first ris-ing and then decreasing",GA3 content showed a trend of"first decreasing and then rising".We suggest that the gene NCED and CYP707A may be the key genes in the dormancy regulation of konjac bulbil.
关键词
珠芽魔芋/温度/休眠/NCED/CYP707AKey words
konjac bulbil/temperature/dormancy/NCED/CYP707A引用本文复制引用
基金项目
珠芽黄魔芋优良品种培育与产业化示范项目(202004AC10001-A06)
出版年
2023
国家科技期刊平台
NSTL
万方数据