荷花PIN基因家族的鉴定及非生物胁迫表达分析
Genome-wide identification and expression pattern of the PIN gene family under abiotic stress in Nelumbo nucifera
程志鹏 1汪仲毅 1匡健华 1赵晗茜 1陈龙清 1胡慧贞1
作者信息
- 1. 西南林业大学园林园艺学院,云南省功能性花卉资源及产业化技术工程研究中心,昆明 650224
- 折叠
摘要
[目的]探究荷花中NnPIN家族成员的特征及在非生物胁迫响应中的作用,为荷花抗逆新品种的选育提供新的基因资源.[方法]采用生物信息学方法对荷花NnPIN家族进行全基因组鉴定,并通过qRT-PCR技术分析了其在低温(4 ℃)、水淹及外源3 mmol/L脱落酸(ABA)处理下的表达模式.[结果](1)荷花基因组中共鉴定出12个具跨膜结构域的NnPIN基因,分别命名为NnPIN1～12,且分布在6条染色体上,并主要定位于质膜和内质网;(2)系统进化分析表明,NnPIN蛋白可根据中央亲水环的长度分为经典型和非经典型2种类型,且同一类型具相似结构;经典型具完整的motif 1～12,而非经典型则缺失中央亲水环部分(motif 6,8,9,12);(3)NnPIN基因在启动子区域具有大量光响应、非生物胁迫和激素响应元件,并特异性地具有厌氧、脱落酸(ABA)、低温等响应元件;(4)NnPIN基因(除NnPIN8外)均正向响应外源ABA和低温胁迫,而NnPIN6和NnPIN7正向响应水淹胁迫,NnPIN1、NnPIN2、NnPIN3、NnPIN4、NnPIN5、NnPIN8、NnPIN9 和 NnPIN12 则负向响应水淹胁迫.[结论]NnPIN基因家族特异性参与荷花非生物逆境胁迫响应.
Abstract
[Objective]To characterize NnPIN gene family in lotus(Nelumbo nucifera)and analyze their roles in abiotic stress,in order to provide genetic resources for the breeding of new lotus varieties with stress resistance.[Methods]This study used bioinformatics methods to conduct a genome-wide identifica-tion of the NnPIN gene family in lotus,and used qRT-PCR to analyze their response to low temperature,flooding,and exogenous application of abscisic acid(ABA).[Results](1)A total of 12 NnPIN genes with transmembrane domains were identified in lotus genome,named NnPIN1-12 respectively,which were distributed on 6 chromosomes,and were mainly located in the plasma membrane and endoplasmic re-ticulum.(2)Phylogenetic analysis showed that NnPIN proteins can be divided into two types:Classical and non-classical based on the length of the central hydrophilic ring,and the same type had a similar struc-ture.The classic type had complete motifs 1 to 12,while the non-classic type missed the central hydro-philic ring(motif 6,8,9,12).(3)Cis-acting element analysis found that the NnPIN genes have a large number of light,abiotic stress and hormone responsive elements in the promoter region,specifically hav-ing anaerobic,ABA,low temperature,and other response elements.(4)The NnPIN genes family,ex-cept NnPIN8,responded positively to exogenous ABA and low temperature stress.In addition,NnPIN6 and NnPIN7 positively responded to flooding stress,while NnPIN1-5,NnPIN8,NnPIN9,and NnPIN12 responded negatively to flooding stress.[Conclusion]The NnPIN gene family members are specifically involved in response to abiotic stress in lotus.
关键词
荷花/PIN基因家族/生物信息学/非生物胁迫/表达模式Key words
lotus/PIN gene family/bioinformatics/abiotic stress/expression pattern引用本文复制引用
基金项目
国家自然科学基金地区科学基金项目(32360070)
云南省科技厅重大科技专项计划(202202AE090028)
云南省高层次人才引进计划高端外国专家专项(YNQR-GDWG-2020-001)
云南省农业基础联合面上项目(202101BD070001-124)
云南省教育厅科学研究基金项目(2023Y0748)
出版年
2024
国家科技期刊平台
NSTL
万方数据