向日葵对列当寄生的生理响应及关键代谢通路分析
Physiological response of sunflowers to broomrape parasitism and analysis of the key metabolic pathways
赵榕 1王猛 1赵雅杰 1朱孔艳 1温玉洁 1包海柱1
作者信息
- 1. 内蒙古农业大学农学院,呼和浩特 010019
- 折叠
摘要
[目的]分析向日葵对列当寄生的生理响应及其相关代谢通路,揭示向日葵对列当寄生的抗性分子机制.[方法]以抗列当向日葵材料(s41)和感列当向日葵材料(s26)为供试材料进行接种列当处理,对正常生长(s41CK,s26CK)和列当寄生胁迫(s41,s26)条件下的4个样本进行转录组测序分析,同时对部分关键代谢物质进行生理验证分析.[结果](1)在列当接种条件下,向日葵高感列当材料的植株高度和根量明显受到抑制,而且已有部分列当出土,而高抗列当材料无明显变化,仅有极少数列当寄生.(2)通过对样品间进行差异基因筛选,得到差异表达基因(DEGs)数量分别为 6 362,6 609,5 490,5 469 个.GO 分析表明,s26-s41、s26CK-s41CK、s26-s26CK、s41-s41CK 4 个比较组参与生物过程的差异基因数量较多,参与细胞组分的差异基因数量次之,参与分子功能的较少;KEGG分析表明列当寄生对向日葵的植物信号转导、次生代谢物的生物合成、脂质代谢、氨基酸代谢、糖代谢有较大的影响.(3)在列当寄生条件下,向日葵抗、感列当材料苯丙氨酸解氨酶、肉桂醇脱氢酶活性及谷胱甘肽、总酚、水杨酸含量均有上升趋势,它们的抗逆系统被激活,但感、抗材料相关生理指标变化趋势有所不同,控制相关生理指标的基因表达情况也不同.[结论]接种列当会明显抑制高感向日葵材料植株高度和根量,而对高抗材料则无明显影响;抗性材料通过增强根部木质素合成、抗氧化酶活性以及调控苯丙烷类代谢、水杨酸信号途径等关键基因表达来提高向日葵植株对列当的抗性.
Abstract
[Objective]To study the physiological response of sunflowers to broomrape parasitism and the related metabolic pathways,as well as understanding the molecular mechanism of sunflower resistance to broomrape parasitism.[Methods]The broomrape-resisant(s41)and-sensitive(s26)sunflowers were used as the testing materials.Transcriptome analysis of 4 samples under the condition of parasitic stress was carried out,and physiological verification analysis of key metabolites was carried out.[Results](1)Under inoculation,the plant height and root number of susceptible materials were affected significantly,and some of them were even unearthed,while the resistant materials had no obvious changes,and only a few were parasitized.(2)The number of DEGs was 6 362,6 609,5 490 and 5 469 by screening the differ-ential genes among the samples.GO analysis showed that the four comparison groups,namely s26-s41,s26CK-s41CK,s26-s26CK,and s41-s41CK,had the most differential genes in biological processes,followed by cellular components,and less in molecular functions.KEGG analysis showed that infection had a great in-fluence on plant signal transduction,biosynthesis of secondary metabolites,lipid metabolism,amino acid metabolism,and sugar metabolism in sunflowers.(3)Physiological analyses found that the phenylalanine deaminase,cinnamyl alcohol dehydrogenase,glutathione,total phenols,and salicylic acid showed an up-ward trend,indicating that the stress tolerant system was activated under parasitism.However,the trend of the physiological indicators of the sensitive and tolerant materials were different.Also,the genes con-trolling the physiological responses were differentially expressed.[Conclusion]It was found that inocula-tion with broomrape significantly affected the plant height and root volume of the susceptible materials,while the resistant materials were not affected.The resistant materials were enhanced in root lignin syn-thesis,antioxidant enzyme activity,and the expression of key genes of phenylpropanoid metabolism,sali-cylic acid and signaling pathway,and hence increased the resistance to broomrape.
关键词
向日葵列当/向日葵/转录组/生理响应/代谢通路Key words
Orobanche cumana/Helianthus annuus/transcriptome/physiological response/metabolic pathway引用本文复制引用
基金项目
内蒙古农业大学科技成果转化专项资金动植物品种选育(培育)项目(YZGC2017003)
内蒙古自治区自然科学基金项目(2019MS03049)
2019年内蒙古"草原英才"工程项目()
内蒙古自治区作物栽培与遗传改良重点实验室和农村部华北黄土高原地区作物栽培科学观测实验站项目(25204120)
出版年
2024
国家科技期刊平台
NSTL
万方数据