QTL epistatic effects and its interaction with environment in Brassica juncea L.
[Objective]The study aims to reveal the molecular mechanism of the formation of seed numbers per pod in Brassica juncea L.and Brassica crops,and lay a foundation for improving the yield and breed-ing of B.juncea.[Methods]In this study,221 recombinant inbred lines(RILs)were engineered using the B.juncea L.We analyzed the additive quantitative trait loci(QTL),additive/additive epistatic effects,and genotype/environment interaction effects on the seed numbers per siliqua in mustard-type rapeseed across five distinct environmental settings.[Results]We identified a total of seven additive QTL associated with the number of seeds per siliqua,predominantly localized on chromosomes A02,A03,A05,A08,B02,and B03 of mustard-type rapeseed.The magnitude of additive effects ranged from-11.642 4 to 4.524 6,among them,qSS2-71 had the highest additive effect and transmission rate,reaching-11.642 4 and 14.44％,respectively.The remaining six additive QTL exhibited less pronounced additive effects and heritabilities.Moreover,the study detected seven pairs of additive/additive QTL interactions influencing the number of seeds per siliqua and their corresponding interactions with the environment.The effects of these epistatic QTL interactions ranged from-4.930 8 to 4.193 6,with the genetic effects of these interactions across different environments was approximately zero.Broad-sense heritability of the number of seeds per siliqua trait was 80.98％,while the narrow-sense heritability was 30.98％.[Conclusion]Although the number of seeds per siliqua in mustard-type rapeseed is influenced by environmental factors,the additive effects governing this trait appear to be minimally impacted by the environment,and the epi-static QTL interactions are not substantial.
Brassica juncea L.number of seeds per siliqueadditive effectsQTL-environment interac-tion effectsepistatic interaction effects
万方数据
维普
