Physicochemical characteristics and anthocyanin components affecting the color of rose petals
[Objective]This study aims to explore the mechanism of flower color formation in rose and to provide references and receptor cultivars for flower breeding by quantitative evaluation and correlation analysis of the physicochemical factors affecting coloration of rose petals.There is of great significance for exploring the mechanism of flower color formation and germplasm innovation.[Methods]8 cultivars of rose with different flower colors were used as experimental materials to measure and compare the physico-chemical indexes such as petal color parameter,pH value of petal cells,metal ion content,total anthocya-nin content,total flavonoids content,and total chlorophyll content,etc.,and the anthocyanin components were quantitatively analyzed.[Results](1)There were significant differences between the physicochemical factors of different flower color of roses,among which the factors such as pH value of petal cells,Fe3+,Ca2+and Al3+content,total anthocyanin content,and total flavonoid content were closely related to the formation of petal color,but the changes in the content of total flavonoids and total anthocyanin played di-rect roles,and the factors such as metal ions and pH value of petal cells affected the flower color by chan-ging the structure of anthocyanidin.(2)The components of anthocyanins in petals of different roses were different.Among them,cyanidin-3,5-diglucoside dominated in the roses and mainly regulated the forma-tion of purplish-red flowers;followed by pelargonidin-3,5-diglucoside,which mainly regulated the forma-tion of orange and red flowers.Yellow flowers contained very little anthocyanin and were mainly regulated by carotenoids.Orange flowers were dually regulated by both anthocyanin and carotenoids,while white flowers contained almost no anthocyanin.Rutin was abundant in all 8 rose cultivars,but there was no sig-nificant correlation with petal formation.(3)Cluster analysis of anthocyanin components in 8 rose petals revealed that the pink-purple cultivar'Lavender Flower Circus'was always clustered in a separate group with high rutin content and small anthocyanin content.[Conclusion]The type and content of anthocyanins directly affect flower color of rose,and flavonoids assist the formation of anthocyanin.Factors such as pH value of petal cells,Fe3+,Ca2+and Al3+content indirectly affect flower color.Among the 8 selected culti-vars of rose,the pink-purple cultivar'Lavender Flower Circus'is the most suitable recipient cultivar for flower color improvement due to its excellent anthocyanin composition and high pH value in petal cells.
Rosa hybridaflower colorphysicochemical characteristicsUPLC-TOF-MS/MSanthocya-nin
万方数据
维普
