摘要
[目的]探索盐胁迫对杠柳幼苗生长及次生代谢物的影响,初步了解杠柳盐耐受范围及盐胁迫对杠柳次生代谢产物的诱导作用.[方法]以培养30 d的杠柳实生苗为材料,测定不同盐浓度[0(CK)、0.2％、0.5％、1.0％、2.0％]处理后,杠柳幼苗的生物量及总甾体成分、强心苷类成分(杠柳毒苷、杠柳次苷及杠柳苷元)、4-甲氧基水杨醛含量的变化特征.[结果]杠柳幼苗在中低浓度盐胁迫下生长良好,且地上部分干鲜质量及地下部分干质量均有不同程度增加,其干湿比也均随盐胁迫程度的增加逐渐升高;植株体内的主要有效成分如总甾体、强心苷类的含量在中等盐胁迫浓度作用下呈现明显上升趋势,而4-甲氧基水杨醛含量则在高浓度盐胁迫下显著增加.[结论]杠柳具有较强的耐盐性,且一定浓度范围内的盐胁迫可显著提高杠柳幼苗根、茎中主要药效成分含量.
Abstract
[Objective]The study aims to explore the effects of salt stress on the growth and secondary me-tabolites of Periploca sepium seedlings,and preliminarily understand the salt tolerance range of P.sepi-um and the induction effect of salt stress on secondary metabolites of P.sepium.[Methods]This experi-ment was conducted with the seedlings of P.sepium cultivated for 30 days to determine the changes in bi-omass,total steroids components,cardiac glycosides(periplocin,periplocymarin,and periplogenin),and 4-methoxy salicylaldehyde content of P.sepium seedlings treated with different salt concentrations(0,0.2％,0.5％,1.0％,2.0％).[Results]The P.sepium seedlings grew well under low concentration of salt stress,and the dry and fresh biomass of the aboveground and underground parts were increased to var-ying degrees.The dry to fresh ratio was gradually increased with the increase in salt stress levels.The content of the major effective components in plants,such as total steroids and cardiac glycosides,showed a significant upward trend under moderate salt stress,while 4-methoxy salicylaldehyde was significantly in-creased under high salt stress.[Conclusion]P.sepium has strong salt tolerance,and salt stress within a certain range increases the content of major secondary metabolites in the roots and stems of P.sepium seedlings.
基金项目
天津市道地药材生态种植及质量保障项目(2022)()
维普
万方数据