Growth and accumulation of the secondary metabolites in Periploca sepium Bunge seedlings under salt stress
[Objective]The study aims to explore the effects of salt stress on the growth and secondary me-tabolites of Periploca sepium seedlings,and preliminarily understand the salt tolerance range of P.sepi-um and the induction effect of salt stress on secondary metabolites of P.sepium.[Methods]This experi-ment was conducted with the seedlings of P.sepium cultivated for 30 days to determine the changes in bi-omass,total steroids components,cardiac glycosides(periplocin,periplocymarin,and periplogenin),and 4-methoxy salicylaldehyde content of P.sepium seedlings treated with different salt concentrations(0,0.2%,0.5%,1.0%,2.0%).[Results]The P.sepium seedlings grew well under low concentration of salt stress,and the dry and fresh biomass of the aboveground and underground parts were increased to var-ying degrees.The dry to fresh ratio was gradually increased with the increase in salt stress levels.The content of the major effective components in plants,such as total steroids and cardiac glycosides,showed a significant upward trend under moderate salt stress,while 4-methoxy salicylaldehyde was significantly in-creased under high salt stress.[Conclusion]P.sepium has strong salt tolerance,and salt stress within a certain range increases the content of major secondary metabolites in the roots and stems of P.sepium seedlings.