[目的]糖外排转运蛋白(sugars will eventually be exported transporters,SWEETs)在植物生长发育过程中发挥重要作用,解析SWEETs基因在枸杞果实发育过程中对糖积累作用,为进一步揭示SWEETs基因在枸杞果实发育过程中的作用提供参考.[方法]用生物信息学方法对枸杞SWEET基因(LbaSWEETs)进行全基因组鉴定,并用已发表的转录数据分析LbaSWEETs在果实发育时期的基因表达情况.[结果]枸杞SWEET基因家族共有37个成员,随机分布于10条染色体上,分别编码152～621个氨基酸,蛋白质分子质量为16.87～69.97 kD,等电点为4.96～9.86.亚细胞定位预测位于叶绿体或质膜,大多数含有7个跨膜螺旋.系统进化分析发现,37个LbaSWEETs蛋白可分为4个亚群,每个亚群的基因结构和保守基序组成相似.启动子元件分析表明:Lba-SWEETs基因启动子富含大量激素响应、逆境胁迫和生长发育响应元件.转录组数据和qRT-PCR分析表明:LbaSWEET9和LbaSWEET29基因表达量随果实成熟呈现显著增加.相关性分析结果表明,LbaSWEET9和LbaSWEET29基因表达量与果糖含量呈显著正相关.[结论]LbaSWEET9和LbaSWEET29基因是果糖积累的关键基因.
Identification of the SWEET gene family and its relationship with soluble sugar content in wolfberry
[Objective]Sugars will eventually be exported transporters(SWEETs)play an important role in growth and development of plants.The aims of the study were to elucidate the role of the SWEET gene family in sugar accumulation during the development of wolfberry,and to provide a reference for revealing the role of the SWEET genes in the development of wolfberry fruits.[Methods]In the present study,based on genomic data of wolfberry,LbaSWEETs were identified by bioinformatics method,and the ex-pression of LbaSWEETs during fruit development was analyzed using published transcriptional data.[Results]There were 37 members of the SWEET gene family,which were randomly distributed on 10 chromosomes,encoding 152-621 amino acids.The molecular weight of the proteins was 16.87-69.97 kD,and the isoelectric point was 4.96-9.86.Subcellular localizations were predicted to be located in the chloroplast or plasma membrane,most containing seven transmembrane helices.Phylogenetic analysis showed that 37 LbaSWEET proteins were divided into 4 subgroups,which had similar gene structures and conserved motifs.Analysis of promoter elements showed that LbaSWEET promoters were rich in hor-mone response,stress response,and growth response elements.Transcriptome and qRT-PCR analysis showed that the expression of LbaSWEET9 and LbaSWEET29 genes was increased significantly with fruit ripening.Pearson correlation analysis showed that the expression levels of LbaSWEET9 and Lba-SWEET29 genes were significantly positively correlated with fructose content.[Conclusion]LbaSWEET9 and LbaSWEET29 are the key genes that may play an important role in the accumulation of fructose sugar.
NETL
NSTL
万方数据
维普
